Literature DB >> 487905

Genetic microsurgery by laser: establishment of a clonal population of rat kangaroo cells (PTK2) with a directed deficiency in a chromosomal nucleolar organizer.

M W Berns, L K Chong, M Hammer-Wilson, K Miller, A Siemens.   

Abstract

An ultraviolet laser beam was focused to a submicron spot on one of the nucleolar organizer regions of mitotic chromosomes of rat kangaroo cells in tissue culture. The daughter cells were isolated and cloned into a viable population that maintained the directed nucleolar deficiency. It is concluded that the laser can be used to delete preselected genetic regions and the genetic deletion is maintained as a heritable deficiency in subsequent daughter cells.

Entities:  

Mesh：

Year:  1979        PMID： 487905     DOI： 10.1007/bf00294839

Source DB:  PubMed          Journal:  Chromosoma        ISSN： 0009-5915            Impact factor:   4.316


  11 in total

1.  Nucleoli and ploidy in Potorous cells (PTK2) in vitro.

Authors:  A D Branch; M W Berns
Journal:  Chromosoma       Date:  1976-06-30       Impact factor: 4.316

2.  Fluorescence analysis of cells using a laser light source.

Authors:  M W Berns
Journal:  Cell Biophys       Date:  1979-03

3.  Directed chromosome loss by laser microirradiation.

Authors:  M W Berns
Journal:  Science       Date:  1974-11-22       Impact factor: 47.728

4.  Modification of nucleolar expression following laser micro-irradiation of chromosomes.

Authors:  M W Berns; Y Ohnuki; D E Rounds; R S Olson
Journal:  Exp Cell Res       Date:  1970-04       Impact factor: 3.905

5.  In vitro production of chromosomal lesions with an argon laser microbeam.

Authors:  M W Berns; R S Olson; D E Rounds
Journal:  Nature       Date:  1969-01-04       Impact factor: 49.962

6.  Laser microirradiation of the nucleolar organizer in cells of the rat kangaroo (Potorous tridactylis). Reduction of nucleolar number and production of micronucleoli.

Authors:  M W Berns; A D Floyd; K Adkisson; W K Cheng; L Moore; G Hoover; K Ustick; S Burgott; T Osial
Journal:  Exp Cell Res       Date:  1972-12       Impact factor: 3.905

7.  The use of proteolytic enzymes for the mapping of structural rearrangements in the chromosomes of man.

Authors:  M Seabright
Journal:  Chromosoma       Date:  1972       Impact factor: 4.316

8.  Are chromosome secondary constrictions nucleolar organizers? A re-examination using a laser microbeam.

Authors:  M W Berns; W K Cheng
Journal:  Exp Cell Res       Date:  1971-11       Impact factor: 3.905

9.  Partial cell irradiation with a tunable organic dye laser.

Authors:  M W Berns
Journal:  Nature       Date:  1972-12-22       Impact factor: 49.962

10.  The absence of centrioles from spindle poles of rat kangaroo (PtK2) cells undergoing meiotic-like reduction division in vitro.

Authors:  S Brenner; A Branch; S Meredith; M W Berns
Journal:  J Cell Biol       Date:  1977-02       Impact factor: 10.539
View more
  8 in total

1.  Gene inactivation by multiphoton-targeted photochemistry.

Authors:  M W Berns; Z Wang; A Dunn; V Wallace; V Venugopalan
Journal:  Proc Natl Acad Sci U S A       Date:  2000-08-15       Impact factor: 11.205

2.  Plant molecular and cellular laser microsurgery.

Authors:  W A Sawahel
Journal:  World J Microbiol Biotechnol       Date:  1996-11       Impact factor: 3.312

3.  Study on mechanism of micronucleoli formation by laser microirradiation.

Authors:  Y H Hu; H Liang; Y Q Jiang
Journal:  Cell Biophys       Date:  1989-06

4.  Direct gene transfer into human cultured cells facilitated by laser micropuncture of the cell membrane.

Authors:  W Tao; J Wilkinson; E J Stanbridge; M W Berns
Journal:  Proc Natl Acad Sci U S A       Date:  1987-06       Impact factor: 11.205

5.  Technique of laser chromosome welding for chromosome repair and artificial chromosome creation.

Authors:  Yao-Xiong Huang; Lin Li; Liu Yang; Yi Zhang
Journal:  Biomed Opt Express       Date:  2018-03-21       Impact factor: 3.732

6.  Induced genetic deficiency of the nucleolar organizer in rat kangaroo cells (PTK1) by ultraviolet laser microirradiation.

Authors:  H Liang; M W Berns
Journal:  Cell Biophys       Date:  1983-03

7.  Analysis of DNA double-strand break response and chromatin structure in mitosis using laser microirradiation.

Authors:  Veronica Gomez-Godinez; Tao Wu; Adria J Sherman; Christopher S Lee; Lih-Huei Liaw; You Zhongsheng; Kyoko Yokomori; Michael W Berns
Journal:  Nucleic Acids Res       Date:  2010-10-04       Impact factor: 16.971

8.  DNA damage induced during mitosis undergoes DNA repair synthesis.

Authors:  Veronica Gomez Godinez; Sami Kabbara; Adria Sherman; Tao Wu; Shirli Cohen; Xiangduo Kong; Jose Luis Maravillas-Montero; Zhixia Shi; Daryl Preece; Kyoko Yokomori; Michael W Berns
Journal:  PLoS One       Date:  2020-04-28       Impact factor: 3.240
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.