Literature DB >> 6190418

Complete analysis of tRNA-modified nucleosides by high-performance liquid chromatography: the 29 modified nucleosides of Salmonella typhimurium and Escherichia coli tRNA.

M Buck, M Connick, B N Ames.   

Abstract

A high-performance liquid chromatography (HPLC) method has been developed to quantify the major and modified nucleoside composition of total, unfractionated transfer RNA. The method is rapid and sensitive and offers a high degree of chromatographic resolution suitable for quantifying both stable and unstable modified nucleosides. It is nondestructive and allows the recovery of nucleosides for further characterization. We apply the method in the analysis of the 29 modified nucleosides in tRNA from Salmonella typhimurium (and Escherichia coli) and show it to be useful in examining changes in the modified nucleoside content of tRNA. Such changes may be important in regulation.

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Year:  1983        PMID： 6190418     DOI： 10.1016/0003-2697(83)90044-1

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  51 in total

1.  Structural alterations of the tRNA(m1G37)methyltransferase from Salmonella typhimurium affect tRNA substrate specificity.

Authors:  J N Li; G R Björk
Journal:  RNA       Date:  1999-03       Impact factor: 4.942

2.  A novel type of RNA editing occurs in the mitochondrial tRNAs of the centipede Lithobius forficatus.

Authors:  D V Lavrov; W M Brown; J L Boore
Journal:  Proc Natl Acad Sci U S A       Date:  2000-12-05       Impact factor: 11.205

3.  A primordial RNA modification enzyme: the case of tRNA (m1A) methyltransferase.

Authors:  Martine Roovers; Johan Wouters; Janusz M Bujnicki; Catherine Tricot; Victor Stalon; Henri Grosjean; Louis Droogmans
Journal:  Nucleic Acids Res       Date:  2004-01-22       Impact factor: 16.971

4.  The spoU gene of Escherichia coli, the fourth gene of the spoT operon, is essential for tRNA (Gm18) 2'-O-methyltransferase activity.

Authors:  B C Persson; G Jäger; C Gustafsson
Journal:  Nucleic Acids Res       Date:  1997-10-15       Impact factor: 16.971

5.  Mechanistic investigations of the pseudouridine synthase RluA using RNA containing 5-fluorouridine.

Authors:  Christopher S Hamilton; Todd M Greco; Caroline A Vizthum; Joy M Ginter; Murray V Johnston; Eugene G Mueller
Journal:  Biochemistry       Date:  2006-10-03       Impact factor: 3.162

6.  Fluorescent pyrimidine ribonucleotide: synthesis, enzymatic incorporation, and utilization.

Authors:  Seergazhi G Srivatsan; Yitzhak Tor
Journal:  J Am Chem Soc       Date:  2007-01-26       Impact factor: 15.419

7.  Identification of two additional members of the tRNA isopentenyltransferase family in Physcomitrella patens.

Authors:  Gunvant Patil; Bjorn Nicander
Journal:  Plant Mol Biol       Date:  2013-05-28       Impact factor: 4.076

8.  The methylthio group (ms2) of N6-(4-hydroxyisopentenyl)-2-methylthioadenosine (ms2io6A) present next to the anticodon contributes to the decoding efficiency of the tRNA.

Authors:  B Esberg; G R Björk
Journal:  J Bacteriol       Date:  1995-04       Impact factor: 3.490

9.  RlmN and Cfr are radical SAM enzymes involved in methylation of ribosomal RNA.

Authors:  Feng Yan; Jacqueline M LaMarre; Rene Röhrich; Jochen Wiesner; Hassan Jomaa; Alexander S Mankin; Danica Galonić Fujimori
Journal:  J Am Chem Soc       Date:  2010-03-24       Impact factor: 15.419

10.  The ms2io6A37 modification of tRNA in Salmonella typhimurium regulates growth on citric acid cycle intermediates.

Authors:  B C Persson; O Olafsson; H K Lundgren; L Hederstedt; G R Björk
Journal:  J Bacteriol       Date:  1998-06       Impact factor: 3.490
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