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Literature DB >> 6190135

The sequence GGCmCGG is resistant to MspI cleavage.

M Busslinger, E deBoer, S Wright, F G Grosveld, R A Flavell.

Abstract

MspI essentially fails to cut the sequence GGCmCGG at enzyme concentrations which give total digestion of CCGG, CmCGG and GGCCGG sites. This result explains why certain sites in mammalian DNA are resistant to both MspI and HpaII and shows that this results from an idiosynchracy of MspI rather than a novel form of DNA methylation at this site in mammalian cells.

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Year: 1983 PMID： 6190135 PMCID： PMC325986 DOI： 10.1093/nar/11.11.3559

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

14 in total

1. Use of restriction enzymes to study eukaryotic DNA methylation: I. The methylation pattern in ribosomal DNA from Xenopus laevis.

Authors: A P Bird; E M Southern
Journal: J Mol Biol Date: 1978-01-05 Impact factor: 5.469

2. DNA methylation in the human gamma delta beta-globin locus in erythroid and nonerythroid tissues.

Authors: L H van der Ploeg; R A Flavell
Journal: Cell Date: 1980-04 Impact factor: 41.582

3. MspI, an isoschizomer of hpaII which cleaves both unmethylated and methylated hpaII sites.

Authors: C Waalwijk; R A Flavell
Journal: Nucleic Acids Res Date: 1978-09 Impact factor: 16.971

4. The 3' untranslated regions of the duplicated human alpha-globin genes are unexpectedly divergent.

Authors: A M Michelson; S H Orkin
Journal: Cell Date: 1980-11 Impact factor: 41.582

5. Specificity of Hpa II and Hae III DNA methylases.

Authors: M B Mann; H O Smith
Journal: Nucleic Acids Res Date: 1977-12 Impact factor: 16.971

6. A novel type of secondary modification of two CCGG residues in the human gamma delta beta-globin gene locus.

Authors: L H van der Ploeg; J Groffen; R A Flavell
Journal: Nucleic Acids Res Date: 1980-10-24 Impact factor: 16.971

7. Nucleotide sequence of the filamentous bacteriophage M13 DNA genome: comparison with phage fd.

Authors: P M van Wezenbeek; T J Hulsebos; J G Schoenmakers
Journal: Gene Date: 1980-10 Impact factor: 3.688

8. The chromosomal arrangement of human alpha-like globin genes: sequence homology and alpha-globin gene deletions.

Authors: J Lauer; C K Shen; T Maniatis
Journal: Cell Date: 1980-05 Impact factor: 41.582

9. Human fetal G gamma- and A gamma-globin genes: complete nucleotide sequences suggest that DNA can be exchanged between these duplicated genes.

Authors: J L Slightom; A E Blechl; O Smithies
Journal: Cell Date: 1980-10 Impact factor: 41.582

10. Effects of methylation on a synthetic polynucleotide: the B--Z transition in poly(dG-m5dC).poly(dG-m5dC).

Authors: M Behe; G Felsenfeld
Journal: Proc Natl Acad Sci U S A Date: 1981-03 Impact factor: 11.205

37 in total

1. Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.

Authors: M McClelland; M Nelson
Journal: Nucleic Acids Res Date: 1992-05-11 Impact factor: 16.971

2. Hypermethylation of human DNA sequences in embryonal carcinoma cells and somatic tissues but not in sperm.

Authors: X Y Zhang; P T Loflin; C W Gehrke; P A Andrews; M Ehrlich
Journal: Nucleic Acids Res Date: 1987-11-25 Impact factor: 16.971

3. Cleavage of methylated CCCGGG sequences containing either N4-methylcytosine or 5-methylcytosine with MspI, HpaII, SmaI, XmaI and Cfr9I restriction endonucleases.

Authors: V Butkus; L Petrauskiene; Z Maneliene; S Klimasauskas; V Laucys; A Janulaitis
Journal: Nucleic Acids Res Date: 1987-09-11 Impact factor: 16.971

4. Site-specific methylation: effect on DNA modification methyltransferases and restriction endonucleases.

Authors: M Nelson; M McClelland
Journal: Nucleic Acids Res Date: 1991-04-25 Impact factor: 16.971

5. Changes in methylation of tissue cultured soybean cells detected by digestion with the restriction enzymes HpaII and MspI.

Authors: H Quemada; E J Roth; K G Lark
Journal: Plant Cell Rep Date: 1987-02 Impact factor: 4.570

10. Human satellite I sequences include a male specific 2.47 kb tandemly repeated unit containing one Alu family member per repeat.

Authors: M Frommer; J Prosser; P C Vincent
Journal: Nucleic Acids Res Date: 1984-03-26 Impact factor: 16.971