Literature DB >> 20817799

Improved method for bacterial cell capture after flow cytometry cell sorting.

D Guillebault1, M Laghdass, P Catala, I Obernosterer, P Lebaron.   

Abstract

Fixed cells with different nucleic acid contents and scatter properties (low nucleic acid [LNA], high nucleic acid 1 [HNA1], and HNA2) were sorted by flow cytometry (FCM). For each sort, 10,000 cells were efficiently captured on poly-l-lysine-coated microplates, resulting in efficient and reproducible PCR amplification.

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Year:  2010        PMID： 20817799      PMCID： PMC2976227          DOI： 10.1128/AEM.00621-10

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  14 in total

1.  PCR bias in ecological analysis: a case study for quantitative Taq nuclease assays in analyses of microbial communities.

Authors:  S Becker; P Böger; R Oehlmann; A Ernst
Journal:  Appl Environ Microbiol       Date:  2000-11       Impact factor: 4.792

2.  Bacterial diversity of metagenomic and PCR libraries from the Delaware River.

Authors:  Matthew T Cottrell; Lisa A Waidner; Liying Yu; David L Kirchman
Journal:  Environ Microbiol       Date:  2005-12       Impact factor: 5.491

3.  Single-cell genomics.

Authors:  Alan Walker; Julian Parkhill
Journal:  Nat Rev Microbiol       Date:  2008-03       Impact factor: 60.633

4.  Impact of lower salinity waters on bacterial heterotrophic production and community structure in the offshore NW Mediterranean Sea.

Authors:  Mélissa Laghdass; Nyree J West; Nicole Batailler; Jocelyne Caparros; Philippe Catala; François Lantoine; Louise Oriol; Philippe Lebaron; Ingrid Obernosterer
Journal:  Environ Microbiol Rep       Date:  2010-12       Impact factor: 3.541

5.  Strategies for overcoming PCR inhibition.

Authors:  Peter Rådström; Charlotta Löfström; Maria Lövenklev; Rickard Knutsson; Petra Wolffs
Journal:  CSH Protoc       Date:  2008-03-01

Review 6.  Inhibition and facilitation of nucleic acid amplification.

Authors:  I G Wilson
Journal:  Appl Environ Microbiol       Date:  1997-10       Impact factor: 4.792

7.  Comparison of blue nucleic acid dyes for flow cytometric enumeration of bacteria in aquatic systems.

Authors:  P Lebaron; N Parthuisot; P Catala
Journal:  Appl Environ Microbiol       Date:  1998-05       Impact factor: 4.792

8.  Flow sorting of microorganisms for molecular analysis.

Authors:  G Wallner; B Fuchs; S Spring; W Beisker; R Amann
Journal:  Appl Environ Microbiol       Date:  1997-11       Impact factor: 4.792

9.  Improved section adhesion for immunocytochemistry using high molecular weight polymers of L-lysine as a slide coating.

Authors:  W M Huang; S J Gibson; P Facer; J Gu; J M Polak
Journal:  Histochemistry       Date:  1983

10.  Evaluating the flow-cytometric nucleic acid double-staining protocol in realistic situations of planktonic bacterial death.

Authors:  Tania Falcioni; Stefano Papa; Josep M Gasol
Journal:  Appl Environ Microbiol       Date:  2008-01-25       Impact factor: 4.792
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  3 in total

1.  Development of defined microbial population standards using fluorescence activated cell sorting for the absolute quantification of S. aureus using real-time PCR.

Authors:  Alice Martinon; Ultan P Cronin; Martin G Wilkinson
Journal:  Mol Biotechnol       Date:  2012-01       Impact factor: 2.695

2.  A magnetic Gram stain for bacterial detection.

Authors:  Ghyslain Budin; Hyun Jung Chung; Hakho Lee; Ralph Weissleder
Journal:  Angew Chem Int Ed Engl       Date:  2012-06-28       Impact factor: 15.336

3.  Xenobiotics shape the physiology and gene expression of the active human gut microbiome.

Authors:  Corinne Ferrier Maurice; Henry Joseph Haiser; Peter James Turnbaugh
Journal:  Cell       Date:  2013-01-17       Impact factor: 41.582
  3 in total

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