Polyclonal antibody-forming cell activation and immunomodulation of the in vitro immune response induced by streptococcal extracellular products.

The capacity of three different extracellular streptococcal products to induce polyclonal activation of precursors of plaque-forming cells (PFC) was investigated. The gamma fraction (pI = 4.2), previously shown to be only weakly mitogenic, was the most potent activator of rabbit and mouse immunoglobulin-secreting cells. The polyclonal stimulation induced by the two other fractions (kappa: pI = 4.8 and epsilon: pI = 10.3), shown to be mitogenic in both systems, was only observed in the rabbit system. Using these fractions, the in vitro immunomodulation of the anti-sheep red blood cell immune response was also investigated. Both gamma and epsilon fractions were shown to possess adjuvant properties, whereas the kappa fraction was a suppressor of the specific immune response. It appears, therefore, that the diversified immunological activities observed with extracellular streptococcal products can be dissociated and belong to different entities.