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Literature DB >> 6184363

In vitro synthesis of subunits of bacterial luciferase in an Escherichia coli system.

J F Evans, S McCracken, C M Miyamoto, E A Meighen, A F Graham.

Abstract

RNA extracted from the luminescent bacterium Vibrio harveyi was translated in an Escherichia coli system. RNA from highly luminescent cells produced both alpha and beta subunits of luciferase in vitro, as confirmed by immunoprecipitation and partial proteolysis. RNA from cells before induction of luminescence did not direct luciferase synthesis.

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Year: 1983 PMID： 6184363 PMCID： PMC217406 DOI： 10.1128/jb.153.1.543-545.1983

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

11 in total

1. Biosynthesis of the coat protein of coliphage f2 by E. coli extracts.

Authors: D NATHANS; G NOTANI; J H SCHWARTZ; N D ZINDER
Journal: Proc Natl Acad Sci U S A Date: 1962-08 Impact factor: 11.205

2. Membrane polypeptides co-induced with the bacterial bioluminescent system.

Authors: Z Ne'eman; S Ulitzur; D Branton; J W Hastings
Journal: J Biol Chem Date: 1977-07-25 Impact factor: 5.157

3. Cell-free transcription and translation of total spinach chloroplast DNA.

Authors: W Bottomley; P R Whitfeld
Journal: Eur J Biochem Date: 1979-01-02

4. Evidence for misreading during tRNA-dependent protein synthesis in vitro.

Authors: W M Holmes; G W Hatfield; E Goldman
Journal: J Biol Chem Date: 1978-05-25 Impact factor: 5.157

5. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

Authors: D W Cleveland; S G Fischer; M W Kirschner; U K Laemmli
Journal: J Biol Chem Date: 1977-02-10 Impact factor: 5.157

6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

7. Cellular control of the synthesis and activity of the bacterial luminescent system.

Authors: K H Nealson; T Platt; J W Hastings
Journal: J Bacteriol Date: 1970-10 Impact factor: 3.490

8. Modulation of ovalbumin synthesis by estradiol-17 beta and actinomycin D as studied in explants of chick oviduct in culture.

Authors: R D Palmiter; T Oka; R T Schimke
Journal: J Biol Chem Date: 1971-02-10 Impact factor: 5.157

In vitro synthesis of subunits of bacterial luciferase in an Escherichia coli system.

1. Biosynthesis of the coat protein of coliphage f2 by E. coli extracts.

2. Membrane polypeptides co-induced with the bacterial bioluminescent system.

3. Cell-free transcription and translation of total spinach chloroplast DNA.

4. Evidence for misreading during tRNA-dependent protein synthesis in vitro.

5. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

7. Cellular control of the synthesis and activity of the bacterial luminescent system.

8. Modulation of ovalbumin synthesis by estradiol-17 beta and actinomycin D as studied in explants of chick oviduct in culture.

9. Size heterogeneity of T2 messenger RNA.

10. Induced polypeptide synthesis during the development of bacterial bioluminescence.

Review 1. Molecular biology of bacterial bioluminescence.

2. Polycistronic mRNAs code for polypeptides of the Vibrio harveyi luminescence system.

3. Expression of bioluminescence by Escherichia coli containing recombinant Vibrio harveyi DNA.