Literature DB >> 6184205

On the mechanism of differential Giemsa staining of BrdU-substituted chromatids.

G González-Gil, M H Navarrete.   

Abstract

This paper analyses the effect of acid hydrolysis on the differential Giemsa staining of 5-bromo-2'deoxyuridine (BrdU) substituted chromatids in human and plant chromosomes, after treatment with a fluorochrome and light. Human lymphocytes and Allium cepa L. root tips were grown in BrdU for two or three cell cycles. Lymphocytes spreadings and meristem squashes were treated with fluorochrome Hoechst 33258, exposed to sunlight, hydrolysed with 5N HCl and stained with giemsa. This acid hydrolysis improves the differential staining of BrdU substituted and non-substituted chromatin. It also allows the differentiation of sister chromatids with the DNA specific dye azure-A.

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Year:  1982        PMID： 6184205     DOI： 10.1007/bf00292264

Source DB:  PubMed          Journal:  Chromosoma        ISSN： 0009-5915            Impact factor:   4.316


  17 in total

1.  ON THE TRANSITION OF DESOXYRIBONUCLEIC ACID TO APURINIC ACID AND THE LOSS OF THE LATTER FROM TISSUES DURING FEULGEN REACTION HYDROLYSIS.

Authors:  J JORDANOV
Journal:  Acta Histochem       Date:  1963-04-30       Impact factor: 2.479

2.  A chemically specific double-staining technique for desoxyribosenucleic acid and other aldehyde-yielding or aldehyde-containing substances.

Authors:  E D DELAMATER; M SCHAECHTER; M E HUNTER
Journal:  J Histochem Cytochem       Date:  1955-01       Impact factor: 2.479

Review 3.  The lesions produced by ultraviolet light in DNA containing 5-bromouracil.

Authors:  F Hutchinson
Journal:  Q Rev Biophys       Date:  1973-05       Impact factor: 5.318

4.  Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes.

Authors:  S A Latt
Journal:  Proc Natl Acad Sci U S A       Date:  1973-12       Impact factor: 11.205

5.  Induction of a high incidence of damage to the X chromosomes of Rattus (Mastomys) natalensis by base analogues, viruses, and carcinogens.

Authors:  C C Huang
Journal:  Chromosoma       Date:  1967       Impact factor: 4.316

6.  Two opposite types of sister chromatid differential staining in BUdR-substituted chromosomes using tetrasodium salt of EDTA.

Authors:  S Takayama; K Tachibana
Journal:  Exp Cell Res       Date:  1980-04       Impact factor: 3.905

7.  Mechanisms of the Feulgen acid hydrolysis.

Authors:  P Kjellstrand
Journal:  J Microsc       Date:  1980-08       Impact factor: 1.758

8.  Giemsa technique for the detection of sister chromatid exchanges.

Authors:  J R Korenberg; E F Freedlender
Journal:  Chromosoma       Date:  1974       Impact factor: 4.316

9.  Simple differential Giemsa staining of sister chromatids after treatment with photosensitive dyes and exposure to light and the mechanism of staining.

Authors:  K Goto; T Akematsu; H Shimazu; T Sugiyama
Journal:  Chromosoma       Date:  1975-12-10       Impact factor: 4.316

10.  Nucleoid condensation and cell division in Escherichia coli MX74T2 ts52 after inhibition of protein synthesis.

Authors:  D R Zusman; A Carbonell; J Y Haga
Journal:  J Bacteriol       Date:  1973-09       Impact factor: 3.490
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  9 in total

1.  In vitro cytogenetic assessment and comparison of vildagliptin and sitagliptin.

Authors:  Ceren Börçek Kasurka; Mehmet Elbistan; Ayşegül Atmaca; Zülal Atlı Şekeroğlu
Journal:  Cytotechnology       Date:  2019-09-25       Impact factor: 2.058

2.  DNA alteration induced by ultraviolet light in human metaphase chromosomes substituted with 5'-bromodeoxy uridine: monitoring by monoclonal antibodies to double-stranded and single stranded DNA.

Authors:  R Mezzanotte; D Peretti; S Orrù; R Rossino; M G Ennas; J Gosalvez
Journal:  Chromosoma       Date:  1989-03       Impact factor: 4.316

3.  DNA lysis is involved in the simplified fluorescence plus Giemsa method for differential staining of sister chromatids.

Authors:  K Y Jan; J L Yang; P F Su
Journal:  Chromosoma       Date:  1984       Impact factor: 4.316

4.  Nuclease activity in human metaphase chromosomes substituted with 5'-bromodeoxyuridine.

Authors:  R Mezzanotte; R Rossino; S Orru; M Mameli; D Peretti
Journal:  Chromosoma       Date:  1989-01       Impact factor: 4.316

5.  Considerations on the mechanism of differential Giemsa staining of BrdU-substituted chromosomes.

Authors:  G Speit
Journal:  Hum Genet       Date:  1984       Impact factor: 4.132

6.  DNA segregation in Escherichia coli cells with 5-bromodeoxyuridine-substituted nucleoids.

Authors:  J L Cánovas; E F Tresguerres; A M Yousif; J F López-Sáez; M H Navarrete
Journal:  J Bacteriol       Date:  1984-04       Impact factor: 3.490

7.  Correlation between tumorigenicity and banding pattern of chromosome 15 in murine T-cell leukemia cells and hybrids of normal and malignant cells.

Authors:  I E Somssich; J Spira; H Hameister; G Klein
Journal:  Chromosoma       Date:  1984       Impact factor: 4.316

8.  Sister chromatid differentiation in 5-bromo-2'-deoxyuridine-substituted chromosomes: a study with DNA-specific ligands and monoclonal antibody to histone H2B.

Authors:  M Ribas; J R Korenberg; D Peretti; G Pichiri; J C Stockert; J Gosalvez; R Mezzanotte
Journal:  Chromosome Res       Date:  1994-11       Impact factor: 5.239

9.  Sister chromatid exchanges in garlic (Allium sativum L.) callus cells.

Authors:  J Doležel; F J Novák
Journal:  Plant Cell Rep       Date:  1986-08       Impact factor: 4.570
  9 in total

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