Literature DB >> 6182288

Trypanosoma cruzi: flow cytometric analysis. I. Analysis of total DNA/organism by means of mithramycin-induced fluorescence.

J A Dvorak, T E Hall, M S Crane, J C Engel, J P McDaniel, R Uriegas.   

Abstract

Total DNA/organism was determined by flow cytometry on stocks of 33 single-cell-isolate clones and one strain of mithramycin-stained Trypanosoma cruzi. Interstrain differences in mean total DNA/group of 34% and interclone differences in total DNA/organism of 41% were found. Microspectrofluorometric analyses of the trypomastigote stage of selected clones confirmed the flow cytometry data and indicated that the total DNA/organism differences were due to differences in DNA of both the nucleus and kinetoplast with the nucleus being the major contributing factor. These data imply that the potential for genetic diversity in T. cruzi may be very large.

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Year:  1982        PMID： 6182288     DOI： 10.1111/j.1550-7408.1982.tb05427.x

Source DB:  PubMed          Journal:  J Protozool        ISSN： 0022-3921


  20 in total

Review 1.  Pathogenesis of chagas' disease: parasite persistence and autoimmunity.

Authors:  Antonio R L Teixeira; Mariana M Hecht; Maria C Guimaro; Alessandro O Sousa; Nadjar Nitz
Journal:  Clin Microbiol Rev       Date:  2011-07       Impact factor: 26.132

2.  Trypanosoma cruzi proliferation and differentiation are blocked by topoisomerase II inhibitors.

Authors:  M Gonzales-Perdomo; S L de Castro; M N Meirelles; S Goldenberg
Journal:  Antimicrob Agents Chemother       Date:  1990-09       Impact factor: 5.191

3.  Characterization and grouping of Trypanosoma cruzi stocks by DNA base-specific fluorochromes and discriminant analysis.

Authors:  H Mühlpfordt; J Berger
Journal:  Parasitol Res       Date:  1990       Impact factor: 2.289

Review 4.  Between a bug and a hard place: Trypanosoma cruzi genetic diversity and the clinical outcomes of Chagas disease.

Authors:  Louisa A Messenger; Michael A Miles; Caryn Bern
Journal:  Expert Rev Anti Infect Ther       Date:  2015-08       Impact factor: 5.091

5.  A stereological study of the differentiation process in Trypanosoma cruzi.

Authors:  M J Soares; T Souto-Padrón; M C Bonaldo; S Goldenberg; W de Souza
Journal:  Parasitol Res       Date:  1989       Impact factor: 2.289

6.  Complete sequence of a 93.4-kb contig from chromosome 3 of Trypanosoma cruzi containing a strand-switch region.

Authors:  B Andersson; L Aslund; M Tammi; A N Tran; J D Hoheisel; U Pettersson
Journal:  Genome Res       Date:  1998-08       Impact factor: 9.043

7.  Identification and detection of Trypanosoma cruzi by using a DNA amplification fingerprint obtained from the ribosomal intergenic spacer.

Authors:  N González; I Galindo; P Guevara; E Novak; J V Scorza; N Añez; J F Da Silveira; J L Ramírez
Journal:  J Clin Microbiol       Date:  1994-01       Impact factor: 5.948

8.  Flow cytometric analysis of the DNA synthetic cycle of Candida species.

Authors:  J A Dvorak; W L Whelan; J P McDaniel; C C Gibson; K J Kwon-Chung
Journal:  Infect Immun       Date:  1987-06       Impact factor: 3.441

9.  Flow cytometric analysis and microsatellite genotyping reveal extensive DNA content variation in Trypanosoma cruzi populations and expose contrasts between natural and experimental hybrids.

Authors:  Michael D Lewis; Martin S Llewellyn; Michael W Gaunt; Matthew Yeo; Hernán J Carrasco; Michael A Miles
Journal:  Int J Parasitol       Date:  2009-04-22       Impact factor: 3.981

10.  Interclonal variations in the molecular karyotype of Trypanosoma cruzi: chromosome rearrangements in a single cell-derived clone of the G strain.

Authors:  Fabio Mitsuo Lima; Renata Torres Souza; Fábio Rinaldo Santori; Michele Fernandes Santos; Danielle Rodrigues Cortez; Roberto Moraes Barros; Maria Isabel Cano; Helder Magno Silva Valadares; Andréa Mara Macedo; Renato Arruda Mortara; José Franco da Silveira
Journal:  PLoS One       Date:  2013-05-07       Impact factor: 3.240
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