| Literature DB >> 6182245 |
Abstract
Polystyrene tubes pretreated with glutaraldehyde in phosphate buffer were used in a solid-phase enzyme-linked immunoassary (ELISA). The hapten o-dinitrocarboxyphenol (o-DNCP) was used as coat. The binding of homologous rabbit antibodies was measured with phosphatase-labelled staphylococcal protein A (SPA-PH). This method is both very sensitive (serum dilutions greater than 2 x 10(-6) were still positive) and highly reproducible. Anti-DNP rat IgE was detected with o-DNCP hapten as a coat using avidin/biotin as a marker.Entities:
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Year: 1982 PMID： 6182245 DOI： 10.1016/0022-1759(82)90244-7
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303