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Literature DB >> 15071032

Genotyping of enteric adenoviruses by using single-stranded conformation polymorphism analysis and heteroduplex mobility assay.

Caroline C Soares¹, Eduardo M Volotão, Maria Carolina M Albuquerque, Carlos M Nozawa, Rosa Elisa C Linhares, Dmitriy Volokhov, Vladimir Chizhikov, Xiaoyan Lu, Dean Erdman, Norma Santos.

Abstract

Single-stranded conformation polymorphism (SSCP) analysis and heteroduplex mobility assays (HMAs) were used to identify and genotype enteric adenoviruses (EAd). The results were compared to those of restriction endonuclease assays, species-specific PCRs, and direct nucleotide sequence analyses. Of the 31 stool samples tested, 15 isolates were identified as EAd and 7 were identified as nonenteric Ad by all methods. An agreement of 100% was found between the SSCP and HMA results.

Mesh：

Substances：
DNA, Viral

Year: 2004 PMID： 15071032 PMCID： PMC387600 DOI： 10.1128/JCM.42.4.1723-1726.2004

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

13 in total

Review 1. Methods for subtyping and molecular comparison of human viral genomes.

Authors: M Arens
Journal: Clin Microbiol Rev Date: 1999-10 Impact factor: 26.132

Review 2. Viral genome characterisation by the heteroduplex mobility and heteroduplex tracking assays.

Authors: K L Barlow; J Green; J P Clewley
Journal: Rev Med Virol Date: 2000 Sep-Oct Impact factor: 6.989

3. PCR and restriction endonuclease analysis for rapid identification of human adenovirus subgenera.

Authors: E M Elnifro; R J Cooper; P E Klapper; A S Bailey
Journal: J Clin Microbiol Date: 2000-06 Impact factor: 5.948

4. A practical approach to genetic screening for influenza virus variants.

Authors: S Zou
Journal: J Clin Microbiol Date: 1997-10 Impact factor: 5.948

5. Use of restriction fragment analysis and sequencing of a serotype-specific region to type adenovirus isolates.

Authors: Q G Li; A Henningsson; P Juto; F Elgh; G Wadell
Journal: J Clin Microbiol Date: 1999-03 Impact factor: 5.948

6. Prevalence of enteric adenoviruses among children with diarrhea in four Brazilian cities.

Authors: Caroline C Soares; Eduardo M Volotão; Maria Carolina M Albuquerque; Fabiano M da Silva; Tatiane R B de Carvalho; Carlos M Nozawa; Rosa E Linhares; Norma Santos
Journal: J Clin Virol Date: 2002-01 Impact factor: 3.168

7. Human immunodeficiency virus type 1 evolution in vivo tracked by DNA heteroduplex mobility assays.

Authors: E L Delwart; H W Sheppard; B D Walker; J Goudsmit; J I Mullins
Journal: J Virol Date: 1994-10 Impact factor: 5.103

8. Adenoviruses from human immunodeficiency virus-infected individuals, including two strains that represent new candidate serotypes Ad50 and Ad51 of species B1 and D, respectively.

Authors: J C De Jong; A G Wermenbol; M W Verweij-Uijterwaal; K W Slaterus; P Wertheim-Van Dillen; G J Van Doornum; S H Khoo; J C Hierholzer
Journal: J Clin Microbiol Date: 1999-12 Impact factor: 5.948

9. Human cytomegalovirus strains associated with congenital and perinatal infections.

Authors: D E Trincado; G M Scott; P A White; C Hunt; L Rasmussen; W D Rawlinson
Journal: J Med Virol Date: 2000-08 Impact factor: 2.327

10. Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels.

Authors: A J Herring; N F Inglis; C K Ojeh; D R Snodgrass; J D Menzies
Journal: J Clin Microbiol Date: 1982-09 Impact factor: 5.948

3 in total