Literature DB >> 6182146

Continuous growth of proximal tubular kidney epithelial cells in hormone-supplemented serum-free medium.

L Chuman, L G Fine, A H Cohen, M H Saier.   

Abstract

An epithelial cell line from pig kidney (LLC-PK1) with properties of proximal tubular cells can be maintained indefinitely in hormone-supplemented serum-free medium. Continuous growth requires the presence of seven factors: transferrin, insulin, selenium, hydrocortisone, triiodothyronine, vasopressin, and cholesterol. The hormone-defined medium (a) supports growth of LLC-PK1 cells at a rate of approaching that observed in serum-supplemented medium; (b) allows vectorial transepithelial salt and fluid transport as measured by hemicyst formation; and (c) influences cell morphology. The vasopressin dependency for growth and morphology can be partially replaced by isobutylmethylxanthine or dibutyryl cyclic AMP. The medium has been used to isolate rabbit proximal tubular kidney epithelial cells free of fibroblasts.

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Year:  1982        PMID： 6182146      PMCID： PMC2112226          DOI： 10.1083/jcb.94.3.506

Source DB:  PubMed          Journal:  J Cell Biol        ISSN： 0021-9525            Impact factor:   10.539


  21 in total

1.  Tight junction formation is closely linked to the polar redistribution of intramembranous particles in aggregating MDCK epithelia.

Authors:  U Hoi Sang; M H Saier; M H Ellisman
Journal:  Exp Cell Res       Date:  1979-09       Impact factor: 3.905

2.  Transepithelial transport in cell culture.

Authors:  D S Misfeldt; S T Hamamoto; D R Pitelka
Journal:  Proc Natl Acad Sci U S A       Date:  1976-04       Impact factor: 11.205

3.  Defined media and the determination of nutritional and hormonal requirements of mammalian cells in culture.

Authors:  A Rizzino; H Rizzino; G Sato
Journal:  Nutr Rev       Date:  1979-12       Impact factor: 7.110

4.  A cell line derived from normal dog kidney (MDCK) exhibiting qualities of papillary adenocarcinoma and of renal tubular epithelium.

Authors:  J Leighton; L W Estes; S Mansukhani; Z Brada
Journal:  Cancer       Date:  1970-11       Impact factor: 6.860

5.  Replacement of serum by hormones permits growth of cells in a defined medium.

Authors:  I Hayashi; G H Sato
Journal:  Nature       Date:  1976-01-15       Impact factor: 49.962

6.  Characterization of an established line of canine kidney cells (MDCK).

Authors:  C R Gaush; W L Hard; T F Smith
Journal:  Proc Soc Exp Biol Med       Date:  1966-07

7.  Growth control of heterologous tissue culture cells in the congenitally athymic nude mouse.

Authors:  C D Stiles; W Desmond; L M Chuman; G Sato; M H Saier
Journal:  Cancer Res       Date:  1976-04       Impact factor: 12.701

8.  Secretory activity and oncogenicity of a cell line (MDCK) derived from canine kidney.

Authors:  J Leighton; Z Brada; L W Estes; G Justh
Journal:  Science       Date:  1969-01-31       Impact factor: 47.728

9.  Regulation of 22Na+ transport by calcium in an established kidney epithelial cell line.

Authors:  M Taub; M H Saier
Journal:  J Biol Chem       Date:  1979-11-25       Impact factor: 5.157

10.  Uptake of 22Na+ by cultured dog kidney cells (MDCK).

Authors:  M J Rindler; M Taub; M H Saier
Journal:  J Biol Chem       Date:  1979-11-25       Impact factor: 5.157
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  26 in total

Review 1.  Serum-free culture of carcinoma cell lines.

Authors:  P Collodi; C Rawson; D Barnes
Journal:  Cytotechnology       Date:  1991-01       Impact factor: 2.058

2.  Primary cultures of normal rat kidney proximal tubule epithelial cells for studies of renal cell injury.

Authors:  K A Elliget; B F Trump
Journal:  In Vitro Cell Dev Biol       Date:  1991-09

Review 3.  Primary kidney cells.

Authors:  M Taub
Journal:  Methods Mol Biol       Date:  1997

Review 4.  Neutral amino acid transport systems in animal cells: potential targets of oncogene action and regulators of cellular growth.

Authors:  M H Saier; G A Daniels; P Boerner; J Lin
Journal:  J Membr Biol       Date:  1988-08       Impact factor: 1.843

5.  Integrated Functional Genomic Analysis Enables Annotation of Kidney Genome-Wide Association Study Loci.

Authors:  Karsten B Sieber; Anna Batorsky; Kyle Siebenthall; Kelly L Hudkins; Jeff D Vierstra; Shawn Sullivan; Aakash Sur; Michelle McNulty; Richard Sandstrom; Alex Reynolds; Daniel Bates; Morgan Diegel; Douglass Dunn; Jemma Nelson; Michael Buckley; Rajinder Kaul; Matthew G Sampson; Jonathan Himmelfarb; Charles E Alpers; Dawn Waterworth; Shreeram Akilesh
Journal:  J Am Soc Nephrol       Date:  2019-02-13       Impact factor: 10.121

Review 6.  Renal epithelial development in organotypic culture.

Authors:  E D Avner; N P Piesco; W E Sweeney; D Ellis
Journal:  Pediatr Nephrol       Date:  1988-01       Impact factor: 3.714

7.  In vitro growth and differentiation of human kidney tubular cells on a basement membrane substrate.

Authors:  A H Yang; J Gould-Kostka; T D Oberley
Journal:  In Vitro Cell Dev Biol       Date:  1987-01

8.  Growth factor requirements of organogenesis in serum-free metanephric organ culture.

Authors:  E D Avner; W E Sweeney; N P Piesco; D Ellis
Journal:  In Vitro Cell Dev Biol       Date:  1985-05

9.  Comprehensive allelotyping of human renal cell carcinomas using microsatellite DNA probes.

Authors:  C A Thrash-Bingham; R E Greenberg; S Howard; A Bruzel; M Bremer; A Goll; H Salazar; J J Freed; K D Tartof
Journal:  Proc Natl Acad Sci U S A       Date:  1995-03-28       Impact factor: 11.205

10.  Differential selenium-dependent expression of type I 5'-deiodinase and glutathione peroxidase in the porcine epithelial kidney cell line LLC-PK1.

Authors:  M Gross; M Oertel; J Köhrle
Journal:  Biochem J       Date:  1995-03-15       Impact factor: 3.857
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