Literature DB >> 6181777

Fractionation of membrane proteins on phenylboronic acid-agarose.

G T Williams, A P Johnstone, P D Dean.   

Abstract

Detergent-solubilized plasma-membrane proteins from lymphocytes of patients with chronic lymphocytic leukaemia were applied to phenylboronic acid-agarose. Some of the polypeptides were bound specifically and subsequently eluted with sorbitol. Immunoprecipitation analyses showed that no surface immunoglobulin M was bound, but that most of the histocompatibility antigens HLA-A, HLA-B, HLA-C and HLA-DR were.

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Year:  1982        PMID： 6181777      PMCID： PMC1158459          DOI： 10.1042/bj2050167

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  15 in total

1.  Isolation of catecholamines in biological fluids by boric acid gel.

Authors:  S Higa; T Suzuki; A Hayashi; I Tsuge; Y Yamamura
Journal:  Anal Biochem       Date:  1977-01       Impact factor: 3.365

2.  Assay of adenylate cyclase by use of polyacrylamide--boronate gel columns.

Authors:  J H Hageman; G D Kuehn
Journal:  Anal Biochem       Date:  1977-06       Impact factor: 3.365

Review 3.  Differentiation antigens of the lymphocyte cell surface.

Authors:  A F Williams
Journal:  Contemp Top Mol Immunol       Date:  1977

Review 4.  Isolation and structure of human histocompatibility (HLA) antigens.

Authors:  M J Crumpton; D Snary
Journal:  Contemp Top Mol Immunol       Date:  1977

Review 5.  Purification of cell membrane glycoproteins by lectin affinity chromatography.

Authors:  R Lotan; G L Nicolson
Journal:  Biochim Biophys Acta       Date:  1979-12-20

6.  Isolation of glycoproteins from pig lymphocyte plasma membrane using Lens culinaris phytohemagglutinin.

Authors:  M J Hayman; M J Crumpton
Journal:  Biochem Biophys Res Commun       Date:  1972-05-26       Impact factor: 3.575

7.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

8.  Sites of nonenzymatic glycosylation of human hemoglobin A.

Authors:  R Shapiro; M J McManus; C Zalut; H F Bunn
Journal:  J Biol Chem       Date:  1980-04-10       Impact factor: 5.157

9.  Purification of ADP-ribosylated nuclear proteins by covalent chromatography on dihydroxyboryl polyacrylamide beads and their characterization.

Authors:  H Okayama; K Ueda; O Hayaishi
Journal:  Proc Natl Acad Sci U S A       Date:  1978-03       Impact factor: 11.205

10.  Activities of lectins and their immobilized derivatives in detergent solutions. Implications on the use of lectin affinity chromatography for the purification of membrane glycoproteins.

Authors:  R Lotan; G Beattie; W Hubbell; G L Nicolson
Journal:  Biochemistry       Date:  1977-05-03       Impact factor: 3.162
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  2 in total

1.  Separation of glycosylated haemoglobins using immobilized phenylboronic acid. Effect of ligand concentration, column operating conditions, and comparison with ion-exchange and isoelectric-focusing.

Authors:  F A Middle; A Bannister; A J Bellingham; P D Dean
Journal:  Biochem J       Date:  1983-03-01       Impact factor: 3.857

2.  Clinical phenotypes combined with saturation genome editing identifying the pathogenicity of BRCA1 variants of uncertain significance in breast cancer.

Authors:  Qiting Wan; Li Hu; Tao Ouyang; Jinfeng Li; Tianfeng Wang; Zhaoqing Fan; Tie Fan; Benyao Lin; Ye Xu; Yuntao Xie
Journal:  Fam Cancer       Date:  2020-08-17       Impact factor: 2.375
  2 in total

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