Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Characterization of an Lyt-1+ cytolytic T-cell clone specific for a polymorphic domain of the I-Ak molecule.

Literature DB >> 6181558

Characterization of an Lyt-1+ cytolytic T-cell clone specific for a polymorphic domain of the I-Ak molecule.

A Pierres, A M Schmitt-Verhulst, M Buferne, P Golstein, M Pierres.

Abstract

We have characterized a cytolytic T-cell clone, isolated from a A.TH anti-A.TL mixed lymphocyte culture, which recognized a private determinant of the I-Ak molecule. This specificity has been confirmed by inhibition of effector-target cell interaction by anti-I-Ak monoclonal antibody (mAb). Comparison of the inhibitory capacity of various mAb and the spatial arrangement of their epitopes (defined in previous studies by antibody-binding competition) indicated that the antigenic site recognized by this cytolytic T-cell clone was topologically related to one of the major polymorphic domains of the Ak molecule. This clone expressed the Thy-1.2+, Lyt-1.+, Lyt-1.2+low and I-As- cell surface phenotype. Testing of several rat mAb, screened for their ability to inhibit H-2K/D-specific cytolysis at the level of the effector cells, revealed that two anti-p94, 180 mAb but not various anti-Lyt-2 mAb inhibited the lytic function of this anti-I-Ak T-cell clone.

Entities: Species

Mesh：

Substances：

Year: 1982 PMID： 6181558 DOI： 10.1111/j.1365-3083.1982.tb00691.x

Source DB: PubMed Journal: Scand J Immunol ISSN： 0300-9475 Impact factor: 3.487

Keyword Cloud
Cited

7 in total

1. T-cell gamma gene is allelically but not isotypically excluded and is not required in known functional T-cell subsets.

Authors: J S Heilig; S Tonegawa
Journal: Proc Natl Acad Sci U S A Date: 1987-11 Impact factor: 11.205

2. Interactions between MHC-encoded products and cloned T-cells. I. Fine specificity of induction of proliferation and lysis.

Authors: F Albert; M Buferne; C Boyer; A M Schmitt-Verhulst
Journal: Immunogenetics Date: 1982 Impact factor: 2.846

3. Dissection of the Poly(Glu60Ala30Tyr10) (GAT)-specific T-cell repertoire in H-2Ik mice. II. The use of monoclonal antibodies to study the recognition of Ia antigens by GAT-reactive T-cell clones.

Authors: P Naquet; S Marchetto; M Pierres
Journal: Immunogenetics Date: 1983 Impact factor: 2.846

7. Differences in antigen presentation to MHC class I-and class II-restricted influenza virus-specific cytolytic T lymphocyte clones.

Authors: L A Morrison; A E Lukacher; V L Braciale; D P Fan; T J Braciale
Journal: J Exp Med Date: 1986-04-01 Impact factor: 14.307

7 in total

Characterization of an Lyt-1+ cytolytic T-cell clone specific for a polymorphic domain of the I-Ak molecule.

1. T-cell gamma gene is allelically but not isotypically excluded and is not required in known functional T-cell subsets.

2. Interactions between MHC-encoded products and cloned T-cells. I. Fine specificity of induction of proliferation and lysis.

3. Dissection of the Poly(Glu60Ala30Tyr10) (GAT)-specific T-cell repertoire in H-2Ik mice. II. The use of monoclonal antibodies to study the recognition of Ia antigens by GAT-reactive T-cell clones.

4. L3T4 but not LFA-1 participates in antigen presentation by Ak-positive L-cell transformants.

5. Self-sparing of long-term in vitro-cloned or uncloned cytotoxic T lymphocytes.

6. Expression of specific cytolytic activity by H-2I region-restricted, influenza virus-specific T lymphocyte clones.

7. Differences in antigen presentation to MHC class I-and class II-restricted influenza virus-specific cytolytic T lymphocyte clones.