Literature DB >> 6179876

Production and partial characterization of monoclonal antibodies to Mycobacterium leprae.

T P Gillis, T M Buchanan.   

Abstract

Monoclonal antibodies to Mycobacterium leprae were produced by the fusion of BALB/c splenocytes and lymph node cells to BALB/c myeloma (NSI/1) cells. Eleven monoclonal antibodies were characterized as to their reactivity with M. leprae and 18 other mycobacterial species by enzyme-linked immunosorbent assay and immunofluorescence. Two monoclonal antibodies reacted only with M. leprae, and the other nine showed unique patterns of reactivity by enzyme-linked immunosorbent assay. One monoclonal antibody (IIH9) reacted with a 68,000-dalton protein present in extracts from M. leprae, M. tuberculosis H37Rv, M. gastri, and M. smegmatis. Potential uses for these antibodies in serological tests and immunochemical analyses are discussed.

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Year:  1982        PMID： 6179876      PMCID： PMC347507          DOI： 10.1128/iai.37.1.172-178.1982

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  21 in total

1.  Immunological studies on leprosy: separation and evaluation of the antigens of Mycobacterium leprae.

Authors:  R G Navalkar; P J Patel; R R Dalvi
Journal:  J Med Microbiol       Date:  1975-05       Impact factor: 2.472

2.  Continuous cultures of fused cells secreting antibody of predefined specificity.

Authors:  G Köhler; C Milstein
Journal:  Nature       Date:  1975-08-07       Impact factor: 49.962

3.  Immunologic studies on leprosy. 2. Antigenic studies of Mycobacterium leprae.

Authors:  R G Navalkar
Journal:  Z Tropenmed Parasitol       Date:  1973-12

4.  Immunochemical identification of a defined antigen of Mycobacterium leprae.

Authors:  S Estrada-Parra
Journal:  Infect Immun       Date:  1972-02       Impact factor: 3.441

5.  Studies on the antigenic specificity of Mycobacterium leprae. II. Purification and immunological characterization of the soluble antigen in leprosy nodules.

Authors:  M Abe; F Minagawa; Y Yoshino; K Okamura
Journal:  Int J Lepr Other Mycobact Dis       Date:  1972 Apr-Jun

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  The immunology of cytoplasmic antigens of mycobacteria.

Authors:  J B Kwapinski; A Alcasid; E H Kwapinski; V Nairn
Journal:  Can J Microbiol       Date:  1972-08       Impact factor: 2.419

8.  Attempts to establish the armadillo (Dasypus novemcinctus Linn.) as a model for the study of leprosy. I. Report of lepromatoid leprosy in an experimentally infected armadillo.

Authors:  W F Kirchheimer; E E Storrs
Journal:  Int J Lepr Other Mycobact Dis       Date:  1971 Jul-Sep

9.  Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion.

Authors:  G Köhler; C Milstein
Journal:  Eur J Immunol       Date:  1976-07       Impact factor: 5.532

10.  BCG antibody profiles in tuberculoid and lepromatous leprosy.

Authors:  N H Axelsen; M Harboe; O Closs; T Godal
Journal:  Infect Immun       Date:  1974-05       Impact factor: 3.441
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  49 in total

1.  Genes for immunodominant protein antigens are highly homologous in Mycobacterium tuberculosis, Mycobacterium africanum, and the vaccine strain Mycobacterium bovis BCG.

Authors:  M C Lu; M H Lien; R E Becker; H C Heine; A M Buggs; D Lipovsek; R Gupta; P W Robbins; C M Grosskinsky; S C Hubbard
Journal:  Infect Immun       Date:  1987-10       Impact factor: 3.441

2.  Production, characterization, and species specificity of five monoclonal antibodies to Mycobacterium tuberculosis.

Authors:  G V Kadival; S D Chaparas
Journal:  J Clin Microbiol       Date:  1987-01       Impact factor: 5.948

3.  Anti-Hsp65 antibodies recognize M proteins of group A streptococci.

Authors:  A Quinn; T M Shinnick; M W Cunningham
Journal:  Infect Immun       Date:  1996-03       Impact factor: 3.441

4.  Results of a World Health Organization-sponsored workshop on monoclonal antibodies to Mycobacterium leprae.

Authors: 
Journal:  Infect Immun       Date:  1985-05       Impact factor: 3.441

5.  Screening of a recombinant mycobacterial DNA library with polyclonal antiserum and molecular weight analysis of expressed antigens.

Authors:  D B Young; L Kent; R A Young
Journal:  Infect Immun       Date:  1987-06       Impact factor: 3.441

6.  Surface-exposed antigenic cleavage fragments of Neisseria gonorrhoeae proteins 1A and IB.

Authors:  S Schmitt; G Layh; T B Buchanan
Journal:  Infect Immun       Date:  1986-12       Impact factor: 3.441

7.  Immunochemical characterization of a protein associated with Mycobacterium leprae cell wall.

Authors:  T P Gillis; R A Miller; D B Young; S R Khanolkar; T M Buchanan
Journal:  Infect Immun       Date:  1985-08       Impact factor: 3.441

8.  Immunochemical characterization of Mycobacterium leprae antigens by the SDS-polyacrylamide gel electrophoresis immunoperoxidase technique (SGIP) using patients' sera.

Authors:  P R Klatser; M M van Rens; T A Eggelte
Journal:  Clin Exp Immunol       Date:  1984-06       Impact factor: 4.330

9.  Immunological characterization of a human homolog of the 65-kilodalton mycobacterial antigen.

Authors:  A K Dudani; R S Gupta
Journal:  Infect Immun       Date:  1989-09       Impact factor: 3.441

10.  Production and characterization of monoclonal antibodies to Mycobacterium tuberculosis, M. bovis (BCG) and M. leprae.

Authors:  A H Kolk; M L Ho; P R Klatser; T A Eggelte; S Kuijper; S de Jonge; J van Leeuwen
Journal:  Clin Exp Immunol       Date:  1984-12       Impact factor: 4.330
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