Literature DB >> 6176682

The stimulus-induced release of 5-hydroxytryptamine and tryptophan from superfused rat brain synaptosomes.

K J Collard, T N Evans, H A Suter, L S Wilkinson.   

Abstract

The synthesis of 5-hydroxytryptamine (5-HT) from 3H-tryptophan in rat forebrain synaptosomes was studied. Synaptosomes which had been preincubated with 3H-tryptophan were perfused with Krebs solution and the release of tryptophan, 5-HT and 5-hydroxyindoleacetic acid (5-HIAA) was determined. K+ depolarization induced a Ca2+ -dependent release of 5-HT and tryptophan but had no effect on 5-HIAA efflux. This finding suggests that the release of tryptophan is unlikely to be a non-specific effect of depolarization.

Entities:  

Mesh：

Substances：

Year:  1982        PMID： 6176682     DOI： 10.1007/BF01243414

Source DB:  PubMed          Journal:  J Neural Transm            Impact factor:   3.575


  13 in total

1.  [The effects of 5,6-dihydroxytryptamine on the transport mechanisms of various neurotransmitters or their precursors at the synaptosomal level in the rat mesencephalon].

Authors:  M F Belin; J C Kouyoumdjian; J Bardakdjian; P Gonnard
Journal:  Biochem Pharmacol       Date:  1975-11-15       Impact factor: 5.858

2.  The isolation of nerve endings from brain: an electron-microscopic study of cell fragments derived by homogenization and centrifugation.

Authors:  E G GRAY; V P WHITTAKER
Journal:  J Anat       Date:  1962-01       Impact factor: 2.610

3.  Newly synthesized norepinephrine accumulation in the readily releasable pool of a purified adrenergic vesicle fraction.

Authors:  R L Klein; T K Harden
Journal:  Life Sci       Date:  1975-01-15       Impact factor: 5.037

4.  A method for concurrent measurement of picomole quantities of acetylcholine, choline, dopamine, norepinephrine, serotonin, 5-hydroxytryptophan, 5-hydroxyindoleacetic acid, tryptophan, tyrosine, glycine, aspartate, glutamate, alanine, and gamma-aminobutyric acid in single tissue samples from different areas of rat central nervous system.

Authors:  J E Smith; J D Lane; P A Shea; W J McBride; M H Aprison
Journal:  Anal Biochem       Date:  1975-03       Impact factor: 3.365

5.  Regional distribution of high-affinity L-tryptophan uptake in rat brain.

Authors:  S H Höckel; W E Müller; U Wollert
Journal:  Neurosci Lett       Date:  1978-04       Impact factor: 3.046

6.  Synaptosomes from forebrains of rats with midbrain raphe lesions: selective reduction of serotonin uptake.

Authors:  M J Kuhar; R H Roth; G K Aghajanian
Journal:  J Pharmacol Exp Ther       Date:  1972-04       Impact factor: 4.030

7.  Preferential release of newly synthesized 3 H-acetylcholine from rat cerebral cortex slices in vitro.

Authors:  P C Molenaar; V J Nickolson; R L Polak
Journal:  Br J Pharmacol       Date:  1973-01       Impact factor: 8.739

8.  The stimulus-induced release of unmetabolized 5-hydroxytryptamine from superfused rat brain synaptosomes.

Authors:  K J Collard; D M Cassidy; M A Pye; R M Taylor
Journal:  J Neurosci Methods       Date:  1981-08       Impact factor: 2.390

9.  Effects of GABA, dopamine, and substance P on the release of newly synthesized 3H-5-hydroxytryptamine from rat substantia nigra in vitro.

Authors:  J C Reubi; P C Emson; T M Jessell; L L Iversen
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  1978-10       Impact factor: 3.000

10.  Serotonin synthesis with rat brain synaptosomes. Effects of serotonin and monoamineoxidase inhibitors.

Authors:  M Karobath
Journal:  Biochem Pharmacol       Date:  1972-05-01       Impact factor: 5.858
View more
  2 in total

1.  Studies on the mechanism by which tryptophan efflux from isolated synaptosomes is stimulated by depolarization.

Authors:  K J Collard; L S Wilkinson; D J Lewis
Journal:  Br J Pharmacol       Date:  1988-02       Impact factor: 8.739

2.  The regulation of 5-hydroxytryptamine release from superfused synaptosomes by 5-hydroxytryptamine and its immediate precursors.

Authors:  H A Suter; K J Collard
Journal:  Neurochem Res       Date:  1983-06       Impact factor: 3.996
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.