Literature DB >> 6174636

The antigen spot test (AST): a highly sensitive assay for the detection of antibodies.

P Herbrink, F J Van Bussel, S O Warnaar.   

Abstract

A method is described for detection of antibodies by means of nitrocellulose or diazobenzyloxymethyl (DBM) paper on which various antigens have been spotted. The sensitivity of this antigen spot test (AST) is comparable with that of RIA and ELISA. The method requires only nanogram amounts of antigen. Since a variety of antigens can be spotted on a single piece of nitrocellulose or DBM paper, this antigen spot test is especially useful for specificity controls on antibodies.

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Year:  1982        PMID： 6174636     DOI： 10.1016/0022-1759(82)90330-1

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  22 in total

1.  Development, characterization, and diagnostic applications of monoclonal antibodies against bovine rotavirus.

Authors:  Y Al-Yousif; F Al-Majhdi; C Chard-Bergstrom; J Anderson; S Kapil
Journal:  Clin Diagn Lab Immunol       Date:  2000-03

2.  Localization of discontinuous epitopes of herpes simplex virus glycoprotein D: use of a nondenaturing ("native" gel) system of polyacrylamide gel electrophoresis coupled with Western blotting.

Authors:  G H Cohen; V J Isola; J Kuhns; P W Berman; R J Eisenberg
Journal:  J Virol       Date:  1986-10       Impact factor: 5.103

3.  Localization of epitopes of herpes simplex virus type 1 glycoprotein D.

Authors:  R J Eisenberg; D Long; M Ponce de Leon; J T Matthews; P G Spear; M G Gibson; L A Lasky; P Berman; E Golub; G H Cohen
Journal:  J Virol       Date:  1985-02       Impact factor: 5.103

4.  Quantitative study of enzyme immunocytochemical reactions performed with enzyme conjugates immobilized on nitrocellulose.

Authors:  P H Nibbering; J G Marijnen; A K Raap; P C Leijh; R van Furth
Journal:  Histochemistry       Date:  1986

5.  Development of an antigen spot test for detection of coronavirus in bovine fecal samples.

Authors:  F Gaber; S Kapil
Journal:  Clin Diagn Lab Immunol       Date:  1999-07

6.  Perceiving mitosis in eukaryotic cells.

Authors:  H Y Kim; D Byrne; P Hwang; S C Thompson; P A Kitos
Journal:  In Vitro Cell Dev Biol       Date:  1988-02

7.  Colour development of immunogold-labelled antibodies for light microscopy.

Authors:  P Fritz; J Hoenes; J Schenk; A Mischlinski; A Grau; J G Saal; H V Tuczek; H Multhaupt; G Pfleiderer
Journal:  Histochemistry       Date:  1986

8.  Enzyme-linked immunofiltration assay for rapid serodiagnosis of syphilis.

Authors:  O E Ijsselmuiden; M M Meinardi; J J van der Sluis; H E Menke; E Stolz; R V van Eijk
Journal:  Eur J Clin Microbiol       Date:  1987-06       Impact factor: 3.267

9.  Localization and synthesis of an antigenic determinant of herpes simplex virus glycoprotein D that stimulates the production of neutralizing antibody.

Authors:  G H Cohen; B Dietzschold; M Ponce de Leon; D Long; E Golub; A Varrichio; L Pereira; R J Eisenberg
Journal:  J Virol       Date:  1984-01       Impact factor: 5.103

10.  Antigenic determinants of the attachment protein of Mycoplasma pneumoniae shared by other pathogenic Mycoplasma species.

Authors:  W A Clyde; P C Hu
Journal:  Infect Immun       Date:  1986-02       Impact factor: 3.441
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