Literature DB >> 61736

Detection of Thiobacillus ferrooxidans in acid mine environments by indirect fluorescent antibody staining.

W A Apel, P R Dugan, J A Filppi, M S Rheins.   

Abstract

An indirect fluorescent antibody (FA) staining technique was developed for the rapid detection of Thiobacillus ferrooxidans. The specificity of the FA stain for T. ferrooxidans was demonstrated with both laboratory and environmental samples. Coal refuse examined by scanning electron microscopy exhibited a rough, porous surface, which was characteristically covered by water-soluble crystals. Significant numbers of T. ferrooxidans were detected in the refuse pores. A positive correlation between numbers of T. ferrooxidans and acid production in coal refuse in the laboratory was demonstrated with the FA technique.

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Year:  1976        PMID： 61736      PMCID： PMC170021          DOI： 10.1128/aem.32.1.159-165.1976

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  17 in total

1.  Acidic mine drainage: the rate-determining step.

Authors:  P C Singer; W Stumm
Journal:  Science       Date:  1970-02-20       Impact factor: 47.728

2.  Study of mycorrhizae by means of fluorescent antibody.

Authors:  E L Schmidt; J A Biesbrock; B B Bohlool; D H Marx
Journal:  Can J Microbiol       Date:  1974-02       Impact factor: 2.419

3.  Aerobic heterotrophic bacteria indigenous to pH 2.8 acid mine water: microscopic examination of acid streamers.

Authors:  P R Dugan; C B MacMillan; R M Pfister
Journal:  J Bacteriol       Date:  1970-03       Impact factor: 3.490

4.  Oxidation of metal sulfides by Thiobacillus ferrooxidans grown on different substrates.

Authors:  M Silver; A E Torma
Journal:  Can J Microbiol       Date:  1974-02       Impact factor: 2.419

5.  Ecology of iron-oxidizing bacteria in pyritic materials associated with coal.

Authors:  R T Belly; T D Brock
Journal:  J Bacteriol       Date:  1974-02       Impact factor: 3.490

6.  Detection of Leptospira in soil and water by immunofluorescence staining.

Authors:  R A Henry; R C Johnson; B B Bohlool; E L Schmidt
Journal:  Appl Microbiol       Date:  1971-05

7.  Nonspecific staining: its control in immunofluorescence examination of soil.

Authors:  B B Bohlool; E L Schmidt
Journal:  Science       Date:  1968-11-29       Impact factor: 47.728

8.  A fluorescent antibody technique for the enumeration of faecal streptococci in water.

Authors:  A P Pugsley; L M Evison
Journal:  J Appl Bacteriol       Date:  1975-02

9.  Autecological study of the chemoautotroph Nitrobacter by immunofluorescence.

Authors:  C B Fliermans; B B Bohlool; E L Schmidt
Journal:  Appl Microbiol       Date:  1974-01

10.  Immunofluorescence approach to the study of the ecology of Thermoplasma acidophilum in coal refuse material.

Authors:  B B Bohlool; T D Brock
Journal:  Appl Microbiol       Date:  1974-07
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  16 in total

1.  Bacteria and acidic drainage from coal refuse: inhibition by sodium lauryl sulfate and sodium benzoate.

Authors:  P R Dugan; W A Apel
Journal:  Appl Environ Microbiol       Date:  1983-07       Impact factor: 4.792

2.  Diversity dynamics of marine bacteria studied by immunofluorescent staining on membrane filters.

Authors:  A B Dahle; M Laake
Journal:  Appl Environ Microbiol       Date:  1982-01       Impact factor: 4.792

3.  An indirect fluorescent antibody staining technique for determining population levels ofThiobacillus ferrooxidans in acid mine drainage waters.

Authors:  J E Gates; K D Pham
Journal:  Microb Ecol       Date:  1979-06       Impact factor: 4.552

4.  Survival and viability of nonculturableEscherichia coli andVibrio cholerae in the estuarine and marine environment.

Authors:  H S Xu; N Roberts; F L Singleton; R W Attwell; D J Grimes; R R Colwell
Journal:  Microb Ecol       Date:  1982-12       Impact factor: 4.552

5.  A Combined Immunofluorescence-DNA-Fluorescence Staining Technique for Enumeration of Thiobacillus ferrooxidans in a Population of Acidophilic Bacteria.

Authors:  G Muyzer; A C de Bruyn; D J Schmedding; P Bos; P Westbroek; G J Kuenen
Journal:  Appl Environ Microbiol       Date:  1987-04       Impact factor: 4.792

6.  Determination of the Number of Respiring Thiobacillus ferrooxidans Cells in Water Samples by Using Combined Fluorescent Antibody-2-(p-Iodophenyl)-3-(p-Nitrophenyl)-5-Phenyltetrazolium Chloride Staining.

Authors:  K H Baker; A L Mills
Journal:  Appl Environ Microbiol       Date:  1982-02       Impact factor: 4.792

7.  Inadequacy of the eucaryote inhibitor cycloheximide in studies of protozoan grazing on bacteria at the freshwater-sediment interface.

Authors:  S C Tremaine; A L Mills
Journal:  Appl Environ Microbiol       Date:  1987-08       Impact factor: 4.792

8.  Specific Dot-Immunobinding Assay for Detection and Enumeration of Thiobacillus ferrooxidans.

Authors:  Renato Arredondo; Carlos A Jerez
Journal:  Appl Environ Microbiol       Date:  1989-08       Impact factor: 4.792

9.  Adenosine 5'-triphosphate formation in Thiobacillus ferrooxidans vesicles by H+ ion gradients comparable to those of environmental conditions.

Authors:  W A Apel; P R Dugan; J H Tuttle
Journal:  J Bacteriol       Date:  1980-04       Impact factor: 3.490

10.  Bacterial community in copper sulfide ores inoculated and leached with solution from a commercial-scale copper leaching plant.

Authors:  R T Espejo; J Romero
Journal:  Appl Environ Microbiol       Date:  1997-04       Impact factor: 4.792
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