Literature DB >> 4880718

Nonspecific staining: its control in immunofluorescence examination of soil.

B B Bohlool, E L Schmidt.   

Abstract

Gelatin preparations were used to treat soil slides prior to addition of fluorescent antibody. Nonspecific staining was avoided, with no detectable interference to specific staining. Gelatin-rhodamine conjugates served to counterstain as well as to prevent nonspecific staining.

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Year:  1968        PMID： 4880718     DOI： 10.1126/science.162.3857.1012

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  52 in total

1.  Strain-specific differences in surface antigens of symbiotic algae.

Authors:  R A Kinzie; G S Chee
Journal:  Appl Environ Microbiol       Date:  1982-11       Impact factor: 4.792

2.  Inoculant Production with Diluted Liquid Cultures of Rhizobium spp. and Autoclaved Peat: Evaluation of Diluents, Rhizobium spp., Peats, Sterility Requirements, Storage, and Plant Effectiveness.

Authors:  P Somasegaran
Journal:  Appl Environ Microbiol       Date:  1985-08       Impact factor: 4.792

3.  Identification and enumeration of marine chroococcoid cyanobacteria by immunofluorescence.

Authors:  L Campbell; E J Carpenter; V J Iacono
Journal:  Appl Environ Microbiol       Date:  1983-09       Impact factor: 4.792

4.  Comparison of solid-phase radioimmunoassays for baculoviruses.

Authors:  A M Crawford; P Faulkner; J Kalmakoff
Journal:  Appl Environ Microbiol       Date:  1978-07       Impact factor: 4.792

5.  Marine ammonia- and nitrite-oxidizing bacteria: serological diversity determined by immunofluorescence in culture and in the environment.

Authors:  B B Ward; A F Carlucci
Journal:  Appl Environ Microbiol       Date:  1985-08       Impact factor: 4.792

6.  Serological and Ecological Characteristics of a Nodule-Dominant Serotype from an Indigenous Soil Population of Rhizobium leguminosarum bv. trifolii.

Authors:  K Leung; K Yap; N Dashti; P J Bottomley
Journal:  Appl Environ Microbiol       Date:  1994-02       Impact factor: 4.792

7.  Diversity dynamics of marine bacteria studied by immunofluorescent staining on membrane filters.

Authors:  A B Dahle; M Laake
Journal:  Appl Environ Microbiol       Date:  1982-01       Impact factor: 4.792

8.  Inoculation response of legumes in relation to the number and effectiveness of indigenous Rhizobium populations.

Authors:  P W Singleton; J W Tavares
Journal:  Appl Environ Microbiol       Date:  1986-05       Impact factor: 4.792

9.  Seasonal changes in numbers and the location of a particular bacterial strain of Alteromonas sp. in seagrass sediments.

Authors:  P W Glazebrook; G Moriarty; A C Hayward; I C Macrae
Journal:  Microb Ecol       Date:  1996-01       Impact factor: 4.552

10.  Predominance of Nonculturable Cells of the Biocontrol Strain Pseudomonas fluorescens CHA0 in the Surface Horizon of Large Outdoor Lysimeters.

Authors:  J Troxler; M Zala; Y Moenne-Loccoz; C Keel; G Defago
Journal:  Appl Environ Microbiol       Date:  1997-10       Impact factor: 4.792
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