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Literature DB >> 6170616

Quantitation of catalase activity by microspectrophotometry after diaminobenzidine staining.

Abstract

The absorbance of the reaction product of catalase staining with diaminobenzidine is linearly proportional to enzyme activity. This is shown in semithin Epon sections of model systems containing serum albumin and catalase from bovine or guinea pig liver. Absorbance measurements were also performed on semithin sections of guinea pig liver, and from these, the activity of cytoplasmic (extraperoxisomal) catalase has been derived.

Entities: Chemical Gene Species

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Year: 1981 PMID： 6170616 DOI： 10.1007/BF00501778

Source DB: PubMed Journal: Histochemistry ISSN： 0301-5564

22 in total

1. The distributional error in microspectrophotometry.

Authors: L ORNSTEIN
Journal: Lab Invest Date: 1952 Impact factor: 5.662

2. Relative efficiencies of a soluble and immobilized two-enzyme system of glucose oxidase and catalase.

Authors: J C Bouin; M T Atallah; H O Hultin
Journal: Biochim Biophys Acta Date: 1976-06-07

3. Visualization of peroxisomes (microbodies) and mitochondria with diaminobenzidine.

Authors: A B Novikoff; S Goldfischer
Journal: J Histochem Cytochem Date: 1969-10 Impact factor: 2.479

4. Theoretical and experimental aspects of enzyme determination in a cytochemical model system of polyacrylamide films containing alkaline phosphatase.

Authors: P Van Duijn; E Pascoe; M Van der Ploeg
Journal: J Histochem Cytochem Date: 1967-08 Impact factor: 2.479

5. Cytochemical detection of catalase with 3,3'-diaminobenzidine. A quantitative reinvestigation of the optimal conditions.

Authors: M LeHir; V Herzog; H D Fahimi
Journal: Histochemistry Date: 1979-11

6. Cytochemical demonstration of extraperoxisomal catalase. II. Liver of rhesus monkey and guinea pig.

Authors: F Roels; W de Coster; S Goldfischer
Journal: J Histochem Cytochem Date: 1977-02 Impact factor: 2.479

7. Cytochemical demonstration of extraperoxisomal catalase. I. Sheep liver.

Authors: F Roels
Journal: J Histochem Cytochem Date: 1976-06 Impact factor: 2.479

8. Distribution of organelles and membranes between hepatocytes and nonhepatocytes in the rat liver parenchyma. A stereological study.

Authors: A Blouin; R P Bolender; E R Weibel
Journal: J Cell Biol Date: 1977-02 Impact factor: 10.539

9. Cytochemical localization of peroxidatic activity of catalase in rat hepatic microbodies (peroxisomes).

Authors: H D Fahimi
Journal: J Cell Biol Date: 1969-11 Impact factor: 10.539

10. The effect of glutaraldehyde on catalase. Biochemical and cytochemical studies with beef liver catalase and rat liver peroxisomes.

Authors: V Herzog; H D Fahimi
Journal: J Cell Biol Date: 1974-01 Impact factor: 10.539

15 in total

1. Comparison of different detection methods in quantitative microdensitometry.

Authors: L Ermert; A C Hocke; H R Duncker; W Seeger; M Ermert
Journal: Am J Pathol Date: 2001-02 Impact factor: 4.307

2. Demonstration of 5'-nucleotidase activity in unfixed cryostat sections of rat liver using a combined light- and electron-microscope procedure.

Authors: J Song; K S Bosch; W Tigchelaar; R J Van Den Munckhof; J P Schellens; C J Van Noorden; W M Frederiks
Journal: Histochem J Date: 1995-11

3. Plateau absorbance measurements: an alternative approach to enzyme activity determination illustrated by the example of alkaline phosphatase.

Authors: P Van Duijn; C J Van Noorden
Journal: Histochem J Date: 1989 Sep-Oct

4. Quantitative study of enzyme immunocytochemical reactions performed with enzyme conjugates immobilized on nitrocellulose.

Authors: P H Nibbering; J G Marijnen; A K Raap; P C Leijh; R van Furth
Journal: Histochemistry Date: 1986

5. On the compartmentalization of catalase, fatty acyl-CoA oxidase and urate oxidase in mammalian livers, and the influence of clofibrate treatment on this microlocalization.

Authors: A Hemsley; M Pegg; D Crane; C Masters
Journal: Mol Cell Biochem Date: 1988-10 Impact factor: 3.396