Literature DB >> 6169708

Cloning and characterization of ribosomal RNA gene of Physarum polycephalum.

Y Sakaki, M Nakamura, H Nomiyama, Y Takagi.   

Abstract

As a first step in studies on the structure and expression of rRNA gene of Physarum polycephalum, the cloning of the gene in Escherichia coli with plasmid vector DNAs was performed and 8 different kinds of clones containing 26S, 19S, and 5.85 rRNA genes were obtained. Using these cloned fragments, the location of these rRNA genes was determined by the Southern hybridization and S1 mapping method. Cloning of rDNA fragments made it possible to analyze the fine structure of the rDNA.

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Year:  1981        PMID： 6169708     DOI： 10.1093/oxfordjournals.jbchem.a133369

Source DB:  PubMed          Journal:  J Biochem        ISSN： 0021-924X            Impact factor:   3.387


  4 in total

1.  Nucleotide sequence of the ribosomal RNA gene of Physarum polycephalum: intron 2 and its flanking regions of the 26S rRNA gene.

Authors:  H Nomiyama; S Kuhara; T Kukita; T Otsuka; Y Sakaki
Journal:  Nucleic Acids Res       Date:  1981-11-11       Impact factor: 16.971

2.  Nucleotide sequence of Physarum polycephalum 5.8S rRNA gene and its flanking regions.

Authors:  T Otsuka; H Nomiyama; Y Sakaki; Y Takagi
Journal:  Nucleic Acids Res       Date:  1982-04-10       Impact factor: 16.971

3.  Direct repeats surrounding the ribosomal RNA genes of Physarum polycephalum.

Authors:  M Hattori; A Ljljana; Y Sakaki
Journal:  Nucleic Acids Res       Date:  1984-02-24       Impact factor: 16.971

4.  Complete nucleotide sequence of the 26S rRNA gene of Physarum polycephalum: its significance in gene evolution.

Authors:  T Otsuka; H Nomiyama; H Yoshida; T Kukita; S Kuhara; Y Sakaki
Journal:  Proc Natl Acad Sci U S A       Date:  1983-06       Impact factor: 11.205
  4 in total

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