Literature DB >> 6169646

Staining technique for peptides of Escherichia coli heat-stable enterotoxin.

L Pesti, K Lukács.   

Abstract

Crude and purified Escherichia coli heat-stable enterotoxin was stained directly in polyacrylamide gel rods after electrophoresis with Coomassie brilliant blue G-250 dissolved in 1 N H2SO4--one-month volume of 10 N KOH-12% trichloroacetic acid. The stained heat-stable toxin bands were partially characterized.

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Year:  1981        PMID： 6169646      PMCID： PMC350801          DOI： 10.1128/iai.33.3.944-947.1981

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  8 in total

1.  DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors:  B J DAVIS
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

2.  Purification and partial characterization of heat-stable enterotoxin of enterotoxigenic Escherichia coli.

Authors:  Y Takeda; T Takeda; T Yano; K Yamamoto; T Miwatani
Journal:  Infect Immun       Date:  1979-09       Impact factor: 3.441

3.  Purification and chemical characterization of the heat-stable enterotoxin produced by porcine strains of enterotoxigenic Escherichia coli.

Authors:  J F Alderete; D C Robertson
Journal:  Infect Immun       Date:  1978-03       Impact factor: 3.441

4.  An improved procedure for protein staining in polyacrylamide gels with a new type of Coomassie Brilliant Blue.

Authors:  W Diezel; G Kopperschläger; E Hofmann
Journal:  Anal Biochem       Date:  1972-08       Impact factor: 3.365

5.  The relationship between two apparently different enterotoxins produced by enteropathogenic strains of Escherichia coli of porcine origin.

Authors:  H W Smith; C L Gyles
Journal:  J Med Microbiol       Date:  1970-08       Impact factor: 2.472

6.  Heat-stable enterotoxins from Escherichia coli P16.

Authors:  M N Burgess; N A Mullan; P M Newsome
Journal:  Infect Immun       Date:  1980-06       Impact factor: 3.441

7.  A new staining technique for proteins in polyacrylamide gels using coomassie brilliant blue G250.

Authors:  R W Blakesley; J A Boezi
Journal:  Anal Biochem       Date:  1977-10       Impact factor: 3.365

8.  Biological evaluation of a methanol-soluble, heat-stable Escherichia coli enterotoxin in infant mice, pigs, rabbits, and calves.

Authors:  M N Burgess; R J Bywater; C M Cowley; N A Mullan; P M Newsome
Journal:  Infect Immun       Date:  1978-08       Impact factor: 3.441
  8 in total

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