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Literature DB >> 6169625

An improved method for Y-body identification and confirmation of a high incidence of YY sperm nuclei.

Abstract

The fluorescence of Y-bodies and brightly fluorescing autosomal heterochromatin in quinacrine mustard stained interphase nuclei treated with distamycin A is more brilliant than in nuclei stained only with quinacrine and mustard. In the present study examinations with the aid of this method in several probands and different tissues are described. The effect of distamycin A is demonstrated to depend on its dose and the time of exposure to it. An exceptionally high frequency of YY sperm nuclei, previously also described by other studies, is confirmed in the present work. A hypothesis explaining the effect of distamycin A in interphase nuclei is discussed.

Entities: Chemical

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Year: 1981 PMID： 6169625 DOI： 10.1007/BF00278701

Source DB: PubMed Journal: Hum Genet ISSN： 0340-6717 Impact factor: 4.132

22 in total

10. [Non-random position of homologous chromosomes (no. 9 and YY) in interphase nuclei of human fibroblasts (author's transl)].

Authors: M Spaeter
Journal: Humangenetik Date: 1975

5 in total

4. Incidence of chromosome 3, 7, 10, 11, 17 and X disomy in mature human sperm nuclei as determined by nonradioactive in situ hybridization.

Authors: M Guttenbach; R Schakowski; M Schmid
Journal: Hum Genet Date: 1994-01 Impact factor: 4.132

5. The chromosome constitution of 1000 human spermatozoa.

Authors: R H Martin; W Balkan; K Burns; A W Rademaker; C C Lin; N L Rudd
Journal: Hum Genet Date: 1983 Impact factor: 4.132

5 in total

An improved method for Y-body identification and confirmation of a high incidence of YY sperm nuclei.

1. Attraction between centric heterochromatin of human chromosomes.

2. Studies on frequency of Y chromatin in human sperm.

Review 3. The spermatozoan genome and fertility.

4. Arrangement of centromeres in mouse cells.

5. Technique for identifying Y chromosomes in human interphase nuclei.

6. Correlation between Y chromosome length and fluorescence intensity of Y chromatin on interphase nuclei.

7. Chromosomal aneuploidy in human spermatozoa.

8. Visualization of nucleolar organizer regions im mammalian chromosomes using silver staining.

9. Inhibition of the development of Q-bands on human chromosomes by netropsin.

10. [Non-random position of homologous chromosomes (no. 9 and YY) in interphase nuclei of human fibroblasts (author's transl)].

1. Determination of Y chromosome aneuploidy in human sperm nuclei by nonradioactive in situ hybridization.

2. Estimation of aneuploidy levels in human spermatozoa using chromosome specific probes and in situ hybridisation.

Review 3. Aspects of evaluation, significance, and evolution of human C-band heteromorphism.

4. Incidence of chromosome 3, 7, 10, 11, 17 and X disomy in mature human sperm nuclei as determined by nonradioactive in situ hybridization.

5. The chromosome constitution of 1000 human spermatozoa.