Literature DB >> 6165014

Partial purification and characterization of human gamma (immune) interferon.

Y K Yip, R H Pang, C Urban, J Vilcek.   

Abstract

Human gamma (immune) interferon (IFN-gamma) was produced in lymphocyte cultures stimulated with a phorbol ester (12-O-tetradecanoylphorbol 13-acetate) and purified phytohemagglutinin. Physicochemical analysis showed that human IFN-gamma is a glycoprotein with an isoelectric point around 8.6 and an apparent molecular weight of 58,000 +/- 3000. A purification process for IFN-gamma was developed consisting of sequential chromatographic separations on controlled-pore glass, concanavalin A-Sepharose, and Bio-Gel P-200. This purification process resulted in an increase in specific activity from about 10(4) (crude culture fluid) to an estimated 10(7) units per mg of protein with a cumulative recovery of about 40% of the IFN activity.

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Year:  1981        PMID： 6165014      PMCID： PMC319179          DOI： 10.1073/pnas.78.3.1601

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  17 in total

1.  Time and dosage dependence of immunoenhancement by murine type II interferon preparations.

Authors:  G Sonnenfeld; A D Mandel; T C Merigan
Journal:  Cell Immunol       Date:  1978-10       Impact factor: 4.868

2.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

3.  Production, partial purification and characterization of human and murine interferons--type II.

Authors:  M Wiranowska-Stewart; L S Lin; I A Braude; W E Stewart
Journal:  Mol Immunol       Date:  1980-05       Impact factor: 4.407

4.  Correlation of physicochemical and antigenic properties of human leukocyte interferon subspecies.

Authors:  E A Havell; Y K Yip; J Vilcek
Journal:  Arch Virol       Date:  1977       Impact factor: 2.574

5.  Amino terminal sequence of the major component of human lymphoblastoid interferon.

Authors:  K C Zoon; M E Smith; P J Bridgen; C B Anfinsen; M W Hunkapiller; L E Hood
Journal:  Science       Date:  1980-02-01       Impact factor: 47.728

6.  Human fibroblast interferon: amino acid analysis and amino terminal amino acid sequence.

Authors:  E Knight; M W Hunkapiller; B D Korant; R W Hardy; L E Hood
Journal:  Science       Date:  1980-02-01       Impact factor: 47.728

7.  Immune and virus-induced interferons may activate cells by different derepressional mechanisms.

Authors:  F Dianzani; M Zucca; A Scupham; J A Georgiades
Journal:  Nature       Date:  1980-01-24       Impact factor: 49.962

8.  Inhibition of murine osteogenic sarcomas by treatment with type I or type II interferon.

Authors:  J L Crane; L A Glasgow; E R Kern; J S Youngner
Journal:  J Natl Cancer Inst       Date:  1978-09       Impact factor: 13.506

9.  Large-scale production and physicochemical characterization of human immune interferon.

Authors:  M P Langford; J A Georgiades; G J Stanton; F Dianzani; H M Johnson
Journal:  Infect Immun       Date:  1979-10       Impact factor: 3.441

10.  Glycosylation of interferons. Effects of tunicamycin on human immune interferon.

Authors:  A Mizrahi; J A O'Malley; W A Carter; A Takatsuki; G Tamura; E Sulkowski
Journal:  J Biol Chem       Date:  1978-11-10       Impact factor: 5.157
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  28 in total

1.  Identification and characterization of a monocyte-derived neutrophil-activating factor in corticosteroid-resistant bronchial asthma.

Authors:  J R Wilkinson; A E Crea; T J Clark; T H Lee
Journal:  J Clin Invest       Date:  1989-12       Impact factor: 14.808

2.  Biosynthetic human interferon treatment of herpetic keratitis.

Authors:  G Smolin
Journal:  Trans Am Ophthalmol Soc       Date:  1983

3.  Radiation inactivation of human gamma-interferon: cellular activation requires two dimers.

Authors:  J A Langer; A Rashidbaigi; G Garotta; E Kempner
Journal:  Proc Natl Acad Sci U S A       Date:  1994-06-21       Impact factor: 11.205

4.  Inhibition of interferon production in human fibroblasts by a tumor promoting phorbol ester.

Authors:  H M Frankfort; J Vilcek
Journal:  Arch Virol       Date:  1982       Impact factor: 2.574

5.  Structure of the human interleukin 2 gene.

Authors:  T Fujita; C Takaoka; H Matsui; T Taniguchi
Journal:  Proc Natl Acad Sci U S A       Date:  1983-12       Impact factor: 11.205

6.  Constitutive production of human interferons by mouse cells with bovine papillomavirus as a vector.

Authors:  R Fukunaga; Y Sokawa; S Nagata
Journal:  Proc Natl Acad Sci U S A       Date:  1984-08       Impact factor: 11.205

7.  Human interferon-gamma enhances the expression of class I and class II major histocompatibility complex products in neoplastic cells more effectively than interferon-alpha and interferon-beta.

Authors:  A Dolei; M R Capobianchi; F Ameglio
Journal:  Infect Immun       Date:  1983-04       Impact factor: 3.441

8.  Partial characterization of gamma (immune) interferon mRNA extracted from human lymphocytes.

Authors:  T Taniguchi; R H Pang; Y K Yip; D Henriksen; J Vilcek
Journal:  Proc Natl Acad Sci U S A       Date:  1981-06       Impact factor: 11.205

9.  Purification of two subspecies of human gamma (immune) interferon.

Authors:  Y K Yip; B S Barrowclough; C Urban; J Vilcek
Journal:  Proc Natl Acad Sci U S A       Date:  1982-03       Impact factor: 11.205

10.  Molecular cloning of human immune interferon cDNA and its expression in eukaryotic cells.

Authors:  R Devos; H Cheroutre; Y Taya; W Degrave; H Van Heuverswyn; W Fiers
Journal:  Nucleic Acids Res       Date:  1982-04-24       Impact factor: 16.971
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