Literature DB >> 6164988

A chemical method to enrich RNA by molecules having 5'-terminal triphosphate groups.

P M Chumakov, M A Grachev, S V Netesov, I N Shatsky.   

Abstract

A method is proposed to enrich RNA molecules having a 5'-terminal triphosphate group. The method is based upon selective chemical modification of 5'-triphosphate groups by an antigen-containing amine followed by affinity chromatography on an absorbent loaded with antibodies specific to this antigen. A purification factor up to 37 may be achieved.

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Year:  1981        PMID： 6164988      PMCID： PMC326773          DOI： 10.1093/nar/9.6.1519

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  9 in total

1.  A rightward promoter to the left of the att site of lambda phage DNA: possible participant in site-specific recombination.

Authors:  V V Kravchenko; S K Vasilenko; M A Grachev
Journal:  Gene       Date:  1979-11       Impact factor: 3.688

2.  The properties of oligonucleotide fragments containing the triphosphorylated 5'-termini of nuclear pre-mRNA from Ehrlich ascites carcinoma cells.

Authors:  G Bajszár; O P Samarina; G P Georgiev
Journal:  Mol Biol Rep       Date:  1976-04       Impact factor: 2.316

3.  Nucleotide sequence of bacteriophage phi X174 DNA.

Authors:  F Sanger; G M Air; B G Barrell; N L Brown; A R Coulson; C A Fiddes; C A Hutchison; P M Slocombe; M Smith
Journal:  Nature       Date:  1977-02-24       Impact factor: 49.962

4.  Localization of the 3' end of Escherichia coli 16 S RNA by electron microscopy of antibody-labelled subunits.

Authors:  I N Shatsky; L V Mochalova; M S Kojouharova; A A Bogdanov; V D Vasiliev
Journal:  J Mol Biol       Date:  1979-10-09       Impact factor: 5.469

5.  [Structure of nuclear pre-mRNA. XIII. Hybridization properties of triphosphorylated 5'-end fragments].

Authors:  N I Peunova; O P Samarina; G P Georgiev
Journal:  Mol Biol (Mosk)       Date:  1980

6.  [Isolation of promotor and terminator fragments of T7 phage DNA from a hydrolysate obtained by exposure to restriction endonuclease BsuR].

Authors:  M A Gravchev; E F Zaĭchikov; V V Kravchenko; A G Pletnev
Journal:  Dokl Akad Nauk SSSR       Date:  1978

7.  Studies on some interactions and reactions of oligonucleotides in aqueous solution.

Authors:  R Naylor; P T Gilham
Journal:  Biochemistry       Date:  1966-08       Impact factor: 3.162

8.  [Molecular weight distribution of hydrolysis products of polyuridylic acid by cobra venom ribonuclease. Preparation of polynucleotides of required molecular weight].

Authors:  L G Bolyreva; A S Butorin; S K Vasilenko
Journal:  Biokhimiia       Date:  1978-09

9.  [Gamma-anilides of nucleoside-5'triphosphates as substrates of DNA-dependent RNA-polymerase of Escherichia coli].

Authors:  M A Grachev; E F Zaĭchikov; A G Pletnev; V V Starikov
Journal:  Mol Biol (Mosk)       Date:  1980 May-Jun
  9 in total

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