Literature DB >> 1272261

The properties of oligonucleotide fragments containing the triphosphorylated 5'-termini of nuclear pre-mRNA from Ehrlich ascites carcinoma cells.

G Bajszár, O P Samarina, G P Georgiev.   

Abstract

Triphosphorylated 5'-end fragments 50-150 nucleotides in length were isolated from nuclear pre-mRNA with the aid of a hydroxyapatite chromatography. They are enriched in U and G (28 and 26%, respectively). About 15% of the fragments isolated from poly(U)+RNA contain poly(U) tracks. Neither poly(A)- nor double-stranded sequences were found. Hybridization experiments in conditions of vast DNA excess demonstrated that the 5'-end fragments contain a low amount of highly repetitive sequences but enriched in sequences hybridizing at C0t 1/2 approximately 100.

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Year:  1976        PMID： 1272261     DOI： 10.1007/BF00366258

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  1 in total

1.  On the structure of transcriptional unit in mammalian cells.

Authors:  G P Georgiev; A P Ryskov; C Coutelle; V L Mantieva; E R Avakyan
Journal:  Biochim Biophys Acta       Date:  1972-01-31
  1 in total
  2 in total

1.  Hybridization properties of sequences adjacent to triphosphorylated 5'-ends of nuclear pre-mRNA from mouse Ehrlich carcinoma.

Authors:  N I Peunova; O P Samarina; G P Georgiev
Journal:  Nucleic Acids Res       Date:  1979-08-10       Impact factor: 16.971

2.  A chemical method to enrich RNA by molecules having 5'-terminal triphosphate groups.

Authors:  P M Chumakov; M A Grachev; S V Netesov; I N Shatsky
Journal:  Nucleic Acids Res       Date:  1981-03-25       Impact factor: 16.971
  2 in total

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