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Literature DB >> 6163770

Human fibroblast interferon. An improved purification.

Abstract

Human fibroblast interferon has been purified 2,900-fold to homogeneity. The purification is achieved in two steps by chromatography on blue Sepharose. The specific activity of the homogeneous interferon is 5 X 10(8) units/mg and the yield of biological activity has ranged from 20-40%. The interferon can exist as a monomer (Mr = 20,000) and as a dimer (Mr - 40,000). The dimer can be converted to the monomer by heating in sodium dodecyl sulfate and thioglycolic acid.

Entities: Chemical Gene Species

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Year: 1981 PMID： 6163770

Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157

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Cited

11 in total

1. 3' end structure of the human (2'-5') oligo A synthetase gene: prediction of two distinct proteins with cell type-specific expression.

Authors: P Benech; G Merlin; M Revel; J Chebath
Journal: Nucleic Acids Res Date: 1985-02-25 Impact factor: 16.971

2. Interferon-induced 56,000 Mr protein and its mRNA in human cells: molecular cloning and partial sequence of the cDNA.

Authors: J Chebath; G Merlin; R Metz; P Benech; M Revel
Journal: Nucleic Acids Res Date: 1983-03-11 Impact factor: 16.971

3. Interferon-dependent induction of mRNA for the major histocompatibility antigens in human fibroblasts and lymphoblastoid cells.

Authors: M Fellous; U Nir; D Wallach; G Merlin; M Rubinstein; M Revel
Journal: Proc Natl Acad Sci U S A Date: 1982-05 Impact factor: 11.205

4. Molecular cloning and sequence of partial cDNA for interferon-induced (2'-5')oligo(A) synthetase mRNA from human cells.

Authors: G Merlin; J Chebath; P Benech; R Metz; M Revel
Journal: Proc Natl Acad Sci U S A Date: 1983-08 Impact factor: 11.205

10. Autogenous production of interferon-beta switches on HLA genes during differentiation of histiocytic lymphoma U937 cells.

Authors: A Yarden; H Shure-Gottlieb; J Chebath; M Revel; A Kimchi
Journal: EMBO J Date: 1984-05 Impact factor: 11.598

Human fibroblast interferon. An improved purification.

1. 3' end structure of the human (2'-5') oligo A synthetase gene: prediction of two distinct proteins with cell type-specific expression.

2. Interferon-induced 56,000 Mr protein and its mRNA in human cells: molecular cloning and partial sequence of the cDNA.

3. Interferon-dependent induction of mRNA for the major histocompatibility antigens in human fibroblasts and lymphoblastoid cells.

4. Molecular cloning and sequence of partial cDNA for interferon-induced (2'-5')oligo(A) synthetase mRNA from human cells.

5. Sepharose-immobilized triazine dyes as adsorbants for human lymphoblastoid interferon purification.

6. Selective reduction of c-myc mRNA in Daudi cells by human beta interferon.

7. Interferon regulates c-myc gene expression in Daudi cells at the post-transcriptional level.

8. Inhibition of interferon secretion in human fibroblast by vinblastine.

9. Purification of two subspecies of human gamma (immune) interferon.

10. Autogenous production of interferon-beta switches on HLA genes during differentiation of histiocytic lymphoma U937 cells.