Literature DB >> 6163645

Simultaneous determination of femtomole quantities of 5-hydroxytryptophan, serotonin and 5-hydroxyindoleacetic acid in brain using HPLC with electrochemical detection.

Z Lacković, M Parenti, N H Neff.   

Abstract

A simple rapid method for the determination of 5-hydroxytryptophan, serotonin (5-hydroxytryptamine), and 5-hydroxyindoleacetic acid in brain is presented. Brain proteins are precipitated with Zn(OH)2. The indoles in the supernatant are separated by HPLC in less than than 8 min on a reverse phase column and detected electrochemically. As little as 38 fmol of hydroxyindole compound can be detected and quantitated. Because the method is rapid and uncomplicated many samples can be processed in a day.

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Year:  1981        PMID： 6163645     DOI： 10.1016/0014-2999(81)90481-7

Source DB:  PubMed          Journal:  Eur J Pharmacol        ISSN： 0014-2999            Impact factor:   4.432


  7 in total

1.  Rat brain concentration of administered amino acids: dependence on time of day for administration.

Authors:  T Eriksson; K Wiesel; A Carlsson
Journal:  J Neural Transm       Date:  1986       Impact factor: 3.575

2.  Diurnal rhythms in rat brain large neutral amino acids (LNAAs), monoamines and monoamine metabolites.

Authors:  L Voog; T Eriksson
Journal:  J Neural Transm Gen Sect       Date:  1992

3.  Clinical effects of fenfluramine in ten autistic subjects.

Authors:  W M Klykylo; D Feldis; D O'Grady; D L Ross; C Halloran
Journal:  J Autism Dev Disord       Date:  1985-12

4.  Central dopamine receptor agonist and antagonist actions of the enantiomers of 3-PPP.

Authors:  S Hjorth; A Carlsson; D Clark; K Svensson; H Wikström; D Sanchez; P Lindberg; U Hacksell; L E Arvidsson; A Johansson
Journal:  Psychopharmacology (Berl)       Date:  1983       Impact factor: 4.530

5.  Biochemical and behavioral changes in rats after prolonged treatment with desipramine: interaction with p-chlorophenylalanine.

Authors:  N Brunello; A Volterra; R Cagiano; G C Ianieri; V Cuomo; G Racagni
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  1985-10       Impact factor: 3.000

6.  Modulation of neuronal serotonin uptake by a putative endogenous ligand of imipramine recognition sites.

Authors:  M L Barbaccia; O Gandolfi; D M Chuang; E Costa
Journal:  Proc Natl Acad Sci U S A       Date:  1983-08       Impact factor: 11.205

7.  Serotonin and 5-hydroxyindoleacetic acid concentrations in individual hypothalamic nuclei and other brain areas of rat.

Authors:  K Oomagari; H Uchimura; T Matsumoto; H Yokoo; M Hirano; J S Kim; T Nakahara
Journal:  Experientia       Date:  1984-11-15
  7 in total

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