Literature DB >> 6159638

Mechanism of translocation: relative arrangement of tRNA and mRNA on the ribosome.

A J Matzke, A Barta, E Kuechler.   

Abstract

AcPhe-tRNAPhe from yeast can be photocross-linked to poly(U) on Escherichia coli ribosomes. The photoreaction occurs at the wybutine base situated next to the 3' side of the anticodon. The kinetics and efficiency of crosslinking of AcPhe-Phe-tRNA are the same at both the acceptor site and the peptidyl site. Therefore, the orientation of wybutine with respect to the mRNA is similar in both the pretranslocational and posttranslocational states. AcPhe-Phe-tRNA crosslinked at the acceptor site can still be translocated to the peptidyl site, demonstrating that tRNA and mRNA are transported together. The experiments support a model of translocation in which the conformation of the anticodon loop of tRNA is similar in both the peptidyl site and the acceptor site.

Entities:  

Mesh：

Substances：

Year:  1980        PMID： 6159638      PMCID： PMC350006          DOI： 10.1073/pnas.77.9.5110

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  28 in total

1.  Factor-free ("non-enzymic") and factor-dependent systems of translation of polyuridylic acid by Escherichia coli ribosomes.

Authors:  L P Gavrilova; O E Kostiashkina; V E Koteliansky; N M Rutkevitch; A S Spirin
Journal:  J Mol Biol       Date:  1976-03-15       Impact factor: 5.469

2.  The conformation of the anticodon loop of yeast tRNAPhe in solution and on ribosomes.

Authors:  O W Odom; B B Craig; B A Hardesty
Journal:  Biopolymers       Date:  1978-12       Impact factor: 2.505

3.  Studies on polypeptide elongation factors from Escherichia coli. II. Purification of factors Tu-guanosine diphosphate, Ts, and Tu-Ts, and crystallization of Tu-guanosine diphosphate and Tu-Ts.

Authors:  K I Arai; M Kawakita; Y Kaziro
Journal:  J Biol Chem       Date:  1972-11-10       Impact factor: 5.157

4.  Frameshift suppression: a nucleotide addition in the anticodon of a glycine transfer RNA.

Authors:  D L Riddle; J Carbon
Journal:  Nat New Biol       Date:  1973-04-25

5.  The stoichiometry of ribosomal translocation.

Authors:  J Modolell; B Cabrer; D Vázquez
Journal:  J Biol Chem       Date:  1973-12-25       Impact factor: 5.157

6.  Studies on polynucleotides. LXXXII. Yeast phenylalanine transfer ribonucleic acid: partial digestion with ribonuclease T-1 and derivation of the total primary structure.

Authors:  U L RajBhandary; S H Chang
Journal:  J Biol Chem       Date:  1968-02-10       Impact factor: 5.157

7.  Molecular mechanics of translation: a reciprocating ratchet mechanism.

Authors:  C Woese
Journal:  Nature       Date:  1970-05-30       Impact factor: 49.962

8.  Photochemical properties of Yt base in aqueous solution.

Authors:  S Paszyc; M Rafalska
Journal:  Nucleic Acids Res       Date:  1979-01       Impact factor: 16.971

9.  Stacking of Crick Wobble pair and Watson-Crick pair: stability rules of G-U pairs at ends of helical stems in tRNAs and the relation to codon-anticodon Wobble interaction.

Authors:  H Mizuno; M Sundaralingam
Journal:  Nucleic Acids Res       Date:  1978-11       Impact factor: 16.971

10.  Yeast phenylalanyl-tRNA synthetase. Affinity and photoaffinity labelling of the stereospecific binding sites.

Authors:  M Baltzinger; F Fasiolo; P Remy
Journal:  Eur J Biochem       Date:  1979-07
View more
  9 in total

Review 1.  How translational accuracy influences reading frame maintenance.

Authors:  P J Farabaugh; G R Björk
Journal:  EMBO J       Date:  1999-03-15       Impact factor: 11.598

Review 2.  A gripping tale of ribosomal frameshifting: extragenic suppressors of frameshift mutations spotlight P-site realignment.

Authors:  John F Atkins; Glenn R Björk
Journal:  Microbiol Mol Biol Rev       Date:  2009-03       Impact factor: 11.056

3.  Calculation of the relative geometry of tRNAs in the ribosome from directed hydroxyl-radical probing data.

Authors:  S Joseph; M L Whirl; D Kondo; H F Noller; R B Altman
Journal:  RNA       Date:  2000-02       Impact factor: 4.942

4.  Orientations of transfer RNA in the ribosomal A and P sites.

Authors:  T R Easterwood; F Major; A Malhotra; S C Harvey
Journal:  Nucleic Acids Res       Date:  1994-09-11       Impact factor: 16.971

Review 5.  Peptide-chain elongation in eukaryotes.

Authors:  C G Proud
Journal:  Mol Biol Rep       Date:  1994-05       Impact factor: 2.316

6.  Conserved 5S rRNA complement to tRNA is not required for protein synthesis.

Authors:  B Pace; E A Matthews; K D Johnson; C R Cantor; N R Pace
Journal:  Proc Natl Acad Sci U S A       Date:  1982-01       Impact factor: 11.205

7.  Effect of ribosome binding and translocation on the anticodon of tRNAPhe as studied by wybutine fluorescence.

Authors:  H Paulsen; J M Robertson; W Wintermeyer
Journal:  Nucleic Acids Res       Date:  1982-04-24       Impact factor: 16.971

8.  Erythromycin, carbomycin, and spiramycin inhibit protein synthesis by stimulating the dissociation of peptidyl-tRNA from ribosomes.

Authors:  J R Menninger; D P Otto
Journal:  Antimicrob Agents Chemother       Date:  1982-05       Impact factor: 5.191

9.  Crosslinking transfer RNA and messenger RNA at the ribosomal decoding region: identification of the site of reaction on the messenger RNA.

Authors:  G Steiner; R Lührmann; E Kuechler
Journal:  Nucleic Acids Res       Date:  1984-11-12       Impact factor: 16.971
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.