Literature DB >> 6159591

An improved direct RNA sequence method; its application to Vicia faba 5.8S ribosomal RNA.

Y Tanaka, T A Dyer, G G Brownlee.   

Abstract

We have developed a direct read-off sequencing procedure, based on the method of Stanley and Vassilenko using E. coli 5S ribosomal RNA as a model compound. Radioactive bands were transferred from an acrylamide gel fractionation in the first dimension onto a DEAE-cellulose thin layer plate. After in situ enzymatic digestion with RNase T2, mononucleoside 3',5'-diphosphates were separated in the second dimension by electrophoresis at pH 2.3. Using this two-dimensional procedure the entire sequence of 163 residues of the previously unknown Vicia faba (broad bean) 5.8S ribosomal RNA was deduced.

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Year:  1980        PMID： 6159591      PMCID： PMC323990          DOI： 10.1093/nar/8.6.1259

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  20 in total

1.  New rapid gel sequencing method for RNA.

Authors:  A Simoncsits; G G Brownlee; R S Brown; J R Rubin; H Guilley
Journal:  Nature       Date:  1977-10-27       Impact factor: 49.962

2.  Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film.

Authors:  R A Laskey; A D Mills
Journal:  FEBS Lett       Date:  1977-10-15       Impact factor: 4.124

3.  The nucleotide sequence of Saccharomyces cerevisiae 5.8 S ribosomal ribonucleic acid.

Authors:  G M Rubin
Journal:  J Biol Chem       Date:  1973-06-10       Impact factor: 5.157

4.  Ribosomal RNA homologies in flowering plants: comparison of the nucleotide sequences in 5.8-S rRNA from broad bean, dwarf bean, tomato, sunflower and rye.

Authors:  J Woledge; M J Corry; P I Payne
Journal:  Biochim Biophys Acta       Date:  1974-05-31

5.  No evidence for tissue-specific sequences of cytoplasmic 5 S and 5.8 S ribosomal RNA's in the broad bean.

Authors:  P I Payne; J Woledge; M J Corry
Journal:  FEBS Lett       Date:  1973-09-15       Impact factor: 4.124

6.  The sequence of 5 s ribosomal ribonucleic acid.

Authors:  G G Brownlee; F Sanger; B G Barrell
Journal:  J Mol Biol       Date:  1968-06-28       Impact factor: 5.469

7.  Isolation and characterization of N6-methyladenosine from Escherichia coli valine transfer RNA.

Authors:  M Saneyoshi; F Harada; S Nishimura
Journal:  Biochim Biophys Acta       Date:  1969-10-22

8.  The sequence of a region of bacteriophage phiX174 DNA coding for parts of genes A and B.

Authors:  N L Brown; M Smith
Journal:  J Mol Biol       Date:  1977-10-15       Impact factor: 5.469

9.  Mapping adenines, guanines, and pyrimidines in RNA.

Authors:  H Donis-Keller; A M Maxam; W Gilbert
Journal:  Nucleic Acids Res       Date:  1977-08       Impact factor: 16.971

10.  Nucleotide sequence relationships between vertebrate 5.8 S ribosomal RNAs.

Authors:  M S Khan; B E Maden
Journal:  Nucleic Acids Res       Date:  1977-07       Impact factor: 16.971
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  24 in total

1.  Transcriptome-Wide Identification of Pseudouridine Modifications Using Pseudo-seq.

Authors:  Thomas M Carlile; Maria F Rojas-Duran; Wendy V Gilbert
Journal:  Curr Protoc Mol Biol       Date:  2015-10-01

2.  Nucleotide sequence of the 17S-25S spacer region from rice rDNA.

Authors:  F Takaiwa; K Oono; M Sugiura
Journal:  Plant Mol Biol       Date:  1985-11       Impact factor: 4.076

3.  GC balance in the internal transcribed spacers ITS 1 and ITS 2 of nuclear ribosomal RNA genes.

Authors:  R A Torres; M Ganal; V Hemleben
Journal:  J Mol Evol       Date:  1990-02       Impact factor: 2.395

4.  Nucleotide sequences of the 5.8S rRNA gene and internal transcribed spacer regions in carrot and broad bean ribosomal DNA.

Authors:  Y Yokota; T Kawata; Y Iida; A Kato; S Tanifuji
Journal:  J Mol Evol       Date:  1989-10       Impact factor: 2.395

5.  The nucleotide sequence of a small (3S) seryl-tRNA (anticodon GCU) from beef heart mitochondria.

Authors:  P Arcari; G G Brownlee
Journal:  Nucleic Acids Res       Date:  1980-11-25       Impact factor: 16.971

6.  A fluorescence-labeling method for sequencing small RNA on polyacrylamide gel.

Authors:  T P Wu; K C Ruan; W Y Liu
Journal:  Nucleic Acids Res       Date:  1996-09-01       Impact factor: 16.971

7.  An evaluation of the phylogenetic position of the dinoflagellate Crypthecodinium cohnii based on 5S rRNA characterization.

Authors:  A G Hinnebusch; L C Klotz; R L Blanken; A R Loeblich
Journal:  J Mol Evol       Date:  1981       Impact factor: 2.395

Review 8.  Mass spectrometry of the fifth nucleoside: a review of the identification of pseudouridine in nucleic acids.

Authors:  Anita Durairaj; Patrick A Limbach
Journal:  Anal Chim Acta       Date:  2008-06-26       Impact factor: 6.558

9.  The distribution and properties of RNA primed initiation sites of DNA synthesis at the replication origin of Escherichia coli chromosome.

Authors:  Y Kohara; N Tohdoh; X W Jiang; T Okazaki
Journal:  Nucleic Acids Res       Date:  1985-10-11       Impact factor: 16.971

10.  The 5.8S RNA gene sequence and the ribosomal repeat of Schizosaccharomyces pombe.

Authors:  J Schaak; J Mao; D Söll
Journal:  Nucleic Acids Res       Date:  1982-05-11       Impact factor: 16.971
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