Literature DB >> 6157602

A biochemical assay for the transcription-antitermination function of the coliphage lambda N gene product.

S Ishii, J S Salstrom, Y Sugino, W Szybalski, F Imamoto.   

Abstract

An in vitro assay has been developed for the antiterminating activity of the N gene product (pN) of bacteriophage lambda, based on the N-dependent stimulation of trp mRNA synthesis from the DNA templates of lambda trp transducing phages. Using this assay system, we show (a) that the stimulation of trp mRNA synthesis by pN requires the cis-dominant nutL site on the lambda chromosome and (b) that pN can participate in vitro in the formation of functional antiterminating transcription complexes.

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Year:  1980        PMID： 6157602     DOI： 10.1016/0378-1119(80)90139-0

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  7 in total

1.  Regulation of the operon encoding ribonucleotide reductase: role of the negative sites in nrd repression.

Authors:  C K Tuggle; J A Fuchs
Journal:  J Bacteriol       Date:  1990-04       Impact factor: 3.490

2.  Efficient modification of E. coli RNA polymerase in vitro by the N gene transcription antitermination protein of bacteriophage lambda.

Authors:  Y Goda; J Greenblatt
Journal:  Nucleic Acids Res       Date:  1985-04-11       Impact factor: 16.971

3.  Abortive termination of bioBFCD RNA synthesized in vitro from the bioABFCD operon of Escherichia coli K-12.

Authors:  S K Nath; A Guha
Journal:  Proc Natl Acad Sci U S A       Date:  1982-03       Impact factor: 11.205

4.  Isolation and characterization of nucleoid proteins from Escherichia coli.

Authors:  K Yamazaki; A Nagata; Y Kano; F Imamoto
Journal:  Mol Gen Genet       Date:  1984

5.  Involvement of the nusB gene products in transcription of Escherichia coli tryptophan operon in vitro.

Authors:  K Kuroki; S Ishii; Y Kano; T Miyashita; K Nishi; F Imamoto
Journal:  Mol Gen Genet       Date:  1982

6.  Evidence that ribosomal protein S10 itself is a cellular component necessary for transcription antitermination by phage lambda N protein.

Authors:  A Das; B Ghosh; S Barik; K Wolska
Journal:  Proc Natl Acad Sci U S A       Date:  1985-06       Impact factor: 11.205

7.  Identification and purification of the N gene product of bacteriophage phi 80.

Authors:  K Kanemoto; S Tanaka; T Miyashita; A Matsushiro
Journal:  Mol Gen Genet       Date:  1986-12
  7 in total

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