Literature DB >> 6156670

Isolation of a serum component that stimulates the spreading of cells in culture.

J G Whateley, P Knox.   

Abstract

A foetal-calf-serum component has been isolated that stimulates cells in culture to adopt typical fibroblast morphology. This "spreading" factor is a glycoprotein with a mol. wt. of approx. 62000 and contains 12% carbohydrate; it has an isoelectric point of 4.8. The method described allows purification to homogeneity as judged by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and analytical isoelectric focusing. Under cell-culture conditions the factor is active at 18 micrograms/ml.

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Year:  1980        PMID： 6156670      PMCID： PMC1161360          DOI： 10.1042/bj1850349

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  18 in total

1.  DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors:  B J DAVIS
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

2.  Growth factors for mammalian cells in culture.

Authors:  I LIEBERMAN; P OVE
Journal:  J Biol Chem       Date:  1959-10       Impact factor: 5.157

3.  The serum dependence of baby hamster kidney cell attachment to a substratum.

Authors:  F Grinnell
Journal:  Exp Cell Res       Date:  1976-02       Impact factor: 3.905

4.  Cell adhesion and spreading factor. Partial purification and properties.

Authors:  F Grinnell; D G Hays; D Minter
Journal:  Exp Cell Res       Date:  1977-11       Impact factor: 3.905

5.  Foetal calf serum and the adhesion of cells in culture [proceedings].

Authors:  P Knox; S Griffiths; J G Whateley
Journal:  Biochem Soc Trans       Date:  1979-10       Impact factor: 5.407

6.  Isolation of a collagen-dependent cell attachment factor.

Authors:  R J Klebe
Journal:  Nature       Date:  1974-07-19       Impact factor: 49.962

7.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

8.  Mammalian cell growth proteins. I. Growth stimulation of fetuin.

Authors:  T T Puck; C A Waldren; C Jones
Journal:  Proc Natl Acad Sci U S A       Date:  1968-01       Impact factor: 11.205

9.  Demonstration of the microheterogeneity of fetuin by electrofocusing.

Authors:  M Pagé
Journal:  Biochim Biophys Acta       Date:  1971-06-29

10.  A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses.

Authors:  W C Russell; C Newman; D H Williamson
Journal:  Nature       Date:  1975-02-06       Impact factor: 49.962
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  9 in total

1.  Isolation of a novel cell-attachment and spreading-promoting protein from human serum.

Authors:  M Vuento; M Korkolainen; P Kuusela; E Hölttä
Journal:  Biochem J       Date:  1985-04-15       Impact factor: 3.857

2.  Studies on cultured fibroblasts from patients with defects of biotin-dependent carboxylation.

Authors:  K Bartlett; H Ng; G Dale; A Green; J V Leonard
Journal:  J Inherit Metab Dis       Date:  1981       Impact factor: 4.982

3.  Cell adhesion to low-Mr proteins extractable from mineralized and soft connective tissues.

Authors:  H J Wong; J E Aubin; S Wasi; J Sodek
Journal:  Biochem J       Date:  1985-11-15       Impact factor: 3.857

4.  Epibolin: a protein of human plasma that supports epithelial cell movement.

Authors:  K S Stenn
Journal:  Proc Natl Acad Sci U S A       Date:  1981-11       Impact factor: 11.205

5.  Routine heat inactivation of serum reduces its capacity to promote cell attachment.

Authors:  D J Giard
Journal:  In Vitro Cell Dev Biol       Date:  1987-10

6.  Characterization of human serum spreading factor with monoclonal antibody.

Authors:  D W Barnes; J Silnutzer; C See; M Shaffer
Journal:  Proc Natl Acad Sci U S A       Date:  1983-03       Impact factor: 11.205

7.  Vitronectin at sites of cell-substrate contact in cultures of rat myotubes.

Authors:  M Baetscher; D W Pumplin; R J Bloch
Journal:  J Cell Biol       Date:  1986-08       Impact factor: 10.539

8.  Cell attachment on replicas of SDS polyacrylamide gels reveals two adhesive plasma proteins.

Authors:  E G Hayman; E Engvall; E A'Hearn; D Barnes; M Pierschbacher; E Ruoslahti
Journal:  J Cell Biol       Date:  1982-10       Impact factor: 10.539

9.  The Extracellular Matrix Environment of Clear Cell Renal Cell Carcinoma Determines Cancer Associated Fibroblast Growth.

Authors:  Kyle H Bond; Takuto Chiba; Kieran P H Wynne; Calvin P H Vary; Sunder Sims-Lucas; Jeannine M Coburn; Leif Oxburgh
Journal:  Cancers (Basel)       Date:  2021-11-23       Impact factor: 6.639
  9 in total

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