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Literature DB >> 2408601

Isolation of a novel cell-attachment and spreading-promoting protein from human serum.

M Vuento, M Korkolainen, P Kuusela, E Hölttä.

Abstract

A protein with potent cell-attachment and spreading-promoting activity was isolated from fibronectin-free human serum. The purification steps included affinity chromatography on heparin-agarose and preparative isoelectric focusing. The purified protein was homogeneous as judged from dodecyl sulphate/polyacrylamide-gel electrophoresis. It had an isoelectric point of 5.0 and an Mr of 52 000. The protein promoted the spreading of Chinese-hamster ovary cells to plastic in a manner similar to that observed with fibronectin.

Entities: Chemical Species

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Year: 1985 PMID： 2408601 PMCID： PMC1144861 DOI： 10.1042/bj2270421

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

15 in total

1. Laminin--a glycoprotein from basement membranes.

Authors: R Timpl; H Rohde; P G Robey; S I Rennard; J M Foidart; G R Martin
Journal: J Biol Chem Date: 1979-10-10 Impact factor: 5.157

2. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors: H Towbin; T Staehelin; J Gordon
Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

Isolation of a novel cell-attachment and spreading-promoting protein from human serum.

1. Laminin--a glycoprotein from basement membranes.

2. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Review 3. Connective tissue structure: cell binding to collagen.

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

5. Isolation of a serum component that stimulates the spreading of cells in culture.

6. Basement membrane glycoprotein laminin binds to heparin.

7. Laminin and fibronectin in cell adhesion: enhanced adhesion of cells from regenerating liver to laminin.

8. Purification of fibronectin from human plasma by affinity chromatography under non-denaturing conditions.

9. Isolation of human serum spreading factor.

10. Immunochemical characterization of human plasma fibronectin.

1. Routine heat inactivation of serum reduces its capacity to promote cell attachment.