Literature DB >> 6156196

Total cellular RNA content: correlation between flow cytometry and ultraviolet spectroscopy.

K D Bauer, L A Dethlefsen.   

Abstract

Total RNA content in Chinese hamster ovary and HeLa-S3 cells determined by ultraviolet spectroscopy is compared with the red fluorescence distribution of acridine orange-stained cells observed by flow cytometry. A correlation coefficient of 0.93 is obtained when these methods of estimating RNA content are compared after various RNAse treatments. These data suggest that acridine orange staining effectively quantitates total cellular RNA content when analyzed by flow cytometry, although DNA is also shown to contribute a low but significant background of red fluorescence.

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Year:  1980        PMID： 6156196     DOI： 10.1177/28.6.6156196

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN： 0022-1554            Impact factor:   2.479


  8 in total

1.  Anchorage-dependent control of muscle-specific gene expression in C2C12 mouse myoblasts.

Authors:  D J Milasincic; J Dhawan; S R Farmer
Journal:  In Vitro Cell Dev Biol Anim       Date:  1996-02       Impact factor: 2.416

2.  Subcompartments of the G1 phase of cell cycle detected by flow cytometry.

Authors:  Z Darzynkiewicz; T Sharpless; L Staiano-Coico; M R Melamed
Journal:  Proc Natl Acad Sci U S A       Date:  1980-11       Impact factor: 11.205

3.  Increased mitochondrial uptake of rhodamine 123 during lymphocyte stimulation.

Authors:  Z Darzynkiewicz; L Staiano-Coico; M R Melamed
Journal:  Proc Natl Acad Sci U S A       Date:  1981-04       Impact factor: 11.205

4.  Acridine orange flow cytometric analysis of renal cell carcinoma. Clinicopathologic implications of RNA content.

Authors:  A K el-Naggar; J G Batsakis; K Teague; G Giacco; V F Guinee; D Swanson
Journal:  Am J Pathol       Date:  1990-08       Impact factor: 4.307

5.  Deep ultraviolet mapping of intracellular protein and nucleic acid in femtograms per pixel.

Authors:  Man C Cheung; James G Evans; Brian McKenna; Daniel J Ehrlich
Journal:  Cytometry A       Date:  2011-07-27       Impact factor: 4.355

6.  Reactions of seven basic fluorochromes with unfixed cells obtained from the salivary glands of the dipteran fly Megaselia scalaris Loew (Phoridae).

Authors:  S K Curtis; R R Cowden; D B Benner
Journal:  Histochemistry       Date:  1986

7.  Human keratinocyte culture. Identification and staging of epidermal cell subpopulations.

Authors:  L Staiano-Coico; P J Higgins; Z Darzynkiewicz; M Kimmel; A B Gottlieb; I Pagan-Charry; M R Madden; J L Finkelstein; J M Hefton
Journal:  J Clin Invest       Date:  1986-02       Impact factor: 14.808

8.  Lymphoma models for B cell activation and tolerance. III. Cell cycle dependence for negative signalling of WEHI-231 B lymphoma cells by anti-mu.

Authors:  D W Scott; D Livnat; C A Pennell; P Keng
Journal:  J Exp Med       Date:  1986-07-01       Impact factor: 14.307
  8 in total

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