Literature DB >> 6153193

Trypsin digestion in immunoperoxidase staining.

N W Hautzer, J F Wittkuhn, W T Mccaughey.   

Abstract

The effect of trypsin digestion on the immunoperoxidase-PAP staining of paraffin sections from normal and abnormal tissue was studied. Trypsin treatment reduced background staining considerably, but enhanced specific staining. Results were, therefore, much easier to read. Possible reasons for this result are discussed.

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Year:  1980        PMID: 6153193     DOI: 10.1177/28.1.6153193

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN: 0022-1554            Impact factor:   2.479


  14 in total

1.  A comparative study on tissue processing procedures for the immunohistochemical investigation of oral mucosal Langerhans cells.

Authors:  A W Barrett; A D Beynon; D J Reid
Journal:  Histochem J       Date:  1994-02

2.  Desmin: its value as a marker of muscle derived tumours using a commercial antibody.

Authors:  M Leader; M Collins; J Patel; K Henry
Journal:  Virchows Arch A Pathol Anat Histopathol       Date:  1987

3.  Microwave fixation provides excellent preservation of tissue, cells and antigens for light and electron microscopy.

Authors:  G R Login; A M Dvorak
Journal:  Histochem J       Date:  1988 Jun-Jul

4.  Immunoradioautographic study of alpha-fetoprotein in hepatoma cells.

Authors:  T Suganuma; M Koura; H Isaka; Y Tsukada; F Murata
Journal:  Histochemistry       Date:  1980

5.  Detection of lymphocytes in malignant gliomas by monoclonal antibodies.

Authors:  K Yasuda; T Alderson; J Phillips; K Sikora
Journal:  J Neurol Neurosurg Psychiatry       Date:  1983-08       Impact factor: 10.154

6.  Light microscopic immunocytochemical demonstration of peroxisomal enzymes in epon sections.

Authors:  J A Litwin; S Yokota; T Hashimoto; H D Fahimi
Journal:  Histochemistry       Date:  1984

7.  Collagenase in the immunohistochemical demonstration of laminin, fibronectin and factor VIII/RAg in nervous tissue after fixation.

Authors:  A Mauro; A Bertolotto; I Germano; G Giaccone; M T Giordana; A Migheli; D Schiffer
Journal:  Histochemistry       Date:  1984

8.  Fibronectin in the synovium of chronic inflammatory joint disease.

Authors:  V Mayston; P I Mapp; P G Davies; P A Revell
Journal:  Rheumatol Int       Date:  1984       Impact factor: 2.631

9.  Cyanogen bromide cleavage of methionine residues as a control method for enkephalin immunocytochemistry.

Authors:  G Jirikowski
Journal:  Histochemistry       Date:  1985

10.  Enzymatic cleavage prior to antibody incubation as a method for neuropeptide immunocytochemistry.

Authors:  R Martin; M Schäfer; K H Voigt
Journal:  Histochemistry       Date:  1982
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