Literature DB >> 6151179

Partial purification and functional expression of brain mRNAs coding for neurotransmitter receptors and voltage-operated channels.

K Sumikawa, I Parker, R Miledi.   

Abstract

Poly(A)+ mRNAs extracted from embryonic chicken brain and from adult rat brain were fractionated on sucrose density gradients. The fractions were subsequently injected into Xenopus oocytes where the mRNA was translated. The products were processed and incorporated into the oocyte membrane where they formed functional neurotransmitter receptors and voltage-operated channels. Different mRNA fractions induced the incorporation of different transmitter receptors and voltage-operated channels into the oocyte membrane. These experiments provide a useful step towards the understanding of the structure and function of neurotransmitter receptors and channels.

Entities:  

Mesh：

Substances：

Year:  1984        PMID： 6151179      PMCID： PMC392280          DOI： 10.1073/pnas.81.24.7994

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  21 in total

1.  A calcium-dependent transient outward current in Xenopus laevis oocytes.

Authors:  R Miledi
Journal:  Proc R Soc Lond B Biol Sci       Date:  1982-07-22

2.  Translation of exogenous messenger RNA coding for nicotinic acetylcholine receptors produces functional receptors in Xenopus oocytes.

Authors:  E A Barnard; R Miledi; K Sumikawa
Journal:  Proc R Soc Lond B Biol Sci       Date:  1982-05-22

3.  The molecular cloning and characterisation of cDNA coding for the alpha subunit of the acetylcholine receptor.

Authors:  K Sumikawa; M Houghton; J C Smith; L Bell; B M Richards; E A Barnard
Journal:  Nucleic Acids Res       Date:  1982-10-11       Impact factor: 16.971

4.  Nucleotide and deduced amino acid sequences of Torpedo californica acetylcholine receptor gamma subunit.

Authors:  T Claudio; M Ballivet; J Patrick; S Heinemann
Journal:  Proc Natl Acad Sci U S A       Date:  1983-02       Impact factor: 11.205

5.  Complete mRNA coding sequence of the acetylcholine binding alpha-subunit of Torpedo marmorata acetylcholine receptor: a model for the transmembrane organization of the polypeptide chain.

Authors:  A Devillers-Thiery; J Giraudat; M Bentaboulet; J P Changeux
Journal:  Proc Natl Acad Sci U S A       Date:  1983-04       Impact factor: 11.205

6.  Synthesis of chick brain GABA receptors by frog oocytes.

Authors:  R Miledi; I Parker; K Sumikawa
Journal:  Proc R Soc Lond B Biol Sci       Date:  1982-11-22

7.  Primary structures of beta- and delta-subunit precursors of Torpedo californica acetylcholine receptor deduced from cDNA sequences.

Authors:  M Noda; H Takahashi; T Tanabe; M Toyosato; S Kikyotani; T Hirose; M Asai; H Takashima; S Inayama; T Miyata; S Numa
Journal:  Nature       Date:  1983-01-20       Impact factor: 49.962

8.  Structural homology of Torpedo californica acetylcholine receptor subunits.

Authors:  M Noda; H Takahashi; T Tanabe; M Toyosato; S Kikyotani; Y Furutani; T Hirose; H Takashima; S Inayama; T Miyata; S Numa
Journal:  Nature       Date:  1983-04-07       Impact factor: 49.962

9.  Cholinergic and catecholaminergic receptors in the Xenopus oocyte membrane.

Authors:  K Kusano; R Miledi; J Stinnakre
Journal:  J Physiol       Date:  1982-07       Impact factor: 5.182

10.  Separate fractions of mRNA from Torpedo electric organ induce chloride channels and acetylcholine receptors in Xenopus oocytes.

Authors:  K Sumikawa; I Parker; T Amano; R Miledi
Journal:  EMBO J       Date:  1984-10       Impact factor: 11.598
View more
  24 in total

Review 1.  Use of Xenopus oocytes for the functional expression of plasma membrane proteins.

Authors:  E Sigel
Journal:  J Membr Biol       Date:  1990-09       Impact factor: 1.843

2.  Oscillatory chloride current evoked by temperature jumps during muscarinic and serotonergic activation in Xenopus oocyte.

Authors:  R Miledi; I Parker; K Sumikawa
Journal:  J Physiol       Date:  1987-02       Impact factor: 5.182

3.  Expressional potency of mRNAs encoding receptors and voltage-activated channels in the postmortem rat brain.

Authors:  D S Ragsdale; R Miledi
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-01       Impact factor: 11.205

4.  Messenger RNA coding for only the alpha subunit of the rat brain Na channel is sufficient for expression of functional channels in Xenopus oocytes.

Authors:  A L Goldin; T Snutch; H Lübbert; A Dowsett; J Marshall; V Auld; W Downey; L C Fritz; H A Lester; R Dunn
Journal:  Proc Natl Acad Sci U S A       Date:  1986-10       Impact factor: 11.205

5.  Properties of single sodium channels translated by Xenopus oocytes after injection with messenger ribonucleic acid.

Authors:  E Sigel
Journal:  J Physiol       Date:  1987-05       Impact factor: 5.182

6.  Discrimination of heterogenous mRNAs encoding strychnine-sensitive glycine receptors in Xenopus oocytes by antisense oligonucleotides.

Authors:  H Akagi; D E Patton; R Miledi
Journal:  Proc Natl Acad Sci U S A       Date:  1989-10       Impact factor: 11.205

7.  Tissue-specific expression of the RI and RII sodium channel subtypes.

Authors:  D Gordon; D Merrick; V Auld; R Dunn; A L Goldin; N Davidson; W A Catterall
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205

8.  Repression of nicotinic acetylcholine receptor expression by antisense RNAs and an oligonucleotide.

Authors:  K Sumikawa; R Miledi
Journal:  Proc Natl Acad Sci U S A       Date:  1988-02       Impact factor: 11.205

9.  Expression of functional neurotransmitter receptors in Xenopus oocytes after injection of human brain membranes.

Authors:  Ricardo Miledi; Fabrizio Eusebi; Ataúlfo Martínez-Torres; Eleonora Palma; Flavia Trettel
Journal:  Proc Natl Acad Sci U S A       Date:  2002-09-17       Impact factor: 11.205

10.  Neurotransmitter receptors and voltage-dependent Ca2+ channels encoded by mRNA from the adult corpus callosum.

Authors:  C Matute; R Miledi
Journal:  Proc Natl Acad Sci U S A       Date:  1993-04-15       Impact factor: 11.205
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.