Literature DB >> 6143576

A rapid and efficient method to enrich SAF-protein from scrapie brains of hamsters.

H Hilmert, H Diringer.   

Abstract

Scrapie hamster brains contain at least 5-10 micrograms of scrapie-associated fibrils (SAF) per brain as estimated by the amount of its major constituent, a protein of about 26 000 daltons (SAF-protein). It can be extracted efficiently by a 10% solution of sarkosyl and can be enriched by differential centrifugation and buffer extraction. Scrapie infectivity, SAF, and SAF-protein copurify.

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Year:  1984        PMID： 6143576     DOI： 10.1007/bf01120313

Source DB:  PubMed          Journal:  Biosci Rep        ISSN： 0144-8463            Impact factor:   3.840


  37 in total

1.  A 54-kDa normal cellular protein may be the precursor of the scrapie agent protease-resistant protein.

Authors:  P E Bendheim; D C Bolton
Journal:  Proc Natl Acad Sci U S A       Date:  1986-04       Impact factor: 11.205

2.  Immunological comparison of scrapie-associated fibrils isolated from animals infected with four different scrapie strains.

Authors:  R J Kascsak; R Rubenstein; P A Merz; R I Carp; N K Robakis; H M Wisniewski; H Diringer
Journal:  J Virol       Date:  1986-09       Impact factor: 5.103

3.  Nuclease treatment results in high specific purification of Creutzfeldt-Jakob disease infectivity with a density characteristic of nucleic acid-protein complexes.

Authors:  T Sklaviadis; A Akowitz; E E Manuelidis; L Manuelidis
Journal:  Arch Virol       Date:  1990       Impact factor: 2.574

4.  Immunohistochemical detection of prion protein in lymphoid tissues of sheep with natural scrapie.

Authors:  L J van Keulen; B E Schreuder; R H Meloen; G Mooij-Harkes; M E Vromans; J P Langeveld
Journal:  J Clin Microbiol       Date:  1996-05       Impact factor: 5.948

5.  Mouse polyclonal and monoclonal antibody to scrapie-associated fibril proteins.

Authors:  R J Kascsak; R Rubenstein; P A Merz; M Tonna-DeMasi; R Fersko; R I Carp; H M Wisniewski; H Diringer
Journal:  J Virol       Date:  1987-12       Impact factor: 5.103

6.  Proteinase K-sensitive disease-associated ovine prion protein revealed by conformation-dependent immunoassay.

Authors:  Alana M Thackray; Lee Hopkins; Raymond Bujdoso
Journal:  Biochem J       Date:  2007-01-15       Impact factor: 3.857

7.  Improvement of PrPSc-detection in mouse spleen early at the preclinical stage of scrapie with collagenase-completed tissue homogenization and Sarkosyl-NaCl extraction of PrPSc.

Authors:  K U Grathwohl; M Horiuchi; N Ishiguro; M Shinagawa
Journal:  Arch Virol       Date:  1996       Impact factor: 2.574

8.  Conservation of infectivity in purified fibrillary extracts of scrapie-infected hamster brain after sequential enzymatic digestion or polyacrylamide gel electrophoresis.

Authors:  P Brown; P P Liberski; A Wolff; D C Gajdusek
Journal:  Proc Natl Acad Sci U S A       Date:  1990-09       Impact factor: 11.205

Review 9.  On the biology of prions.

Authors:  S B Prusiner; R Gabizon; M P McKinley
Journal:  Acta Neuropathol       Date:  1987       Impact factor: 17.088

Review 10.  "Clusters" of CJD in Slovakia: the first laboratory evidence of scrapie.

Authors:  E Mitrová; S Huncaga; G Hocman; O Nyitrayová; M Tatara
Journal:  Eur J Epidemiol       Date:  1991-09       Impact factor: 8.082
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