Literature DB >> 2119503

Conservation of infectivity in purified fibrillary extracts of scrapie-infected hamster brain after sequential enzymatic digestion or polyacrylamide gel electrophoresis.

P Brown1, P P Liberski, A Wolff, D C Gajdusek.   

Abstract

Infectious extracts of scrapie-infected hamster brain enriched for scrapie-associated fibrils and scrapie amyloid protein (PrP) were partially denatured and subjected to either polyacrylamide gel electrophoresis with subsequent isolation of the PrP band or sequential enzymatic digestion with deglycosidase, phospholipase, proteinase, and several different nucleases. Infectivity measurements of these various specimens revealed a convincing association between infectivity and scrapie amyloid protein, with or without its sugar chains and disulfide bonds, and did not support the hypothesis that nucleic acid is involved in replication.

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Year:  1990        PMID： 2119503      PMCID： PMC54719          DOI： 10.1073/pnas.87.18.7240

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  42 in total

1.  Molecular hybridization studies with scrapie brain nucleic acids. II. Differential expression in scrapie hamster brain.

Authors:  T Borrás; J J Merendino; C J Gibbs
Journal:  Arch Virol       Date:  1986       Impact factor: 2.574

2.  Molecular hybridization studies with scrapie brain nucleic acids. I. Search for specific DNA sequences.

Authors:  T Borrás; C J Gibbs
Journal:  Arch Virol       Date:  1986       Impact factor: 2.574

3.  Further studies of the infectivity and stability of extracts and homogenates derived from scrapie affected mouse brains.

Authors:  G D Hunter; R A Gibbons; R H Kimberlin; G C Millson
Journal:  J Comp Pathol       Date:  1969-01       Impact factor: 1.311

4.  Diagnosis of Creutzfeldt-Jakob disease by Western blot identification of marker protein in human brain tissue.

Authors:  P Brown; M Coker-Vann; K Pomeroy; M Franko; D M Asher; C J Gibbs; D C Gajdusek
Journal:  N Engl J Med       Date:  1986-02-27       Impact factor: 91.245

5.  Infection-specific particle from the unconventional slow virus diseases.

Authors:  P A Merz; R G Rohwer; R Kascsak; H M Wisniewski; R A Somerville; C J Gibbs; D C Gajdusek
Journal:  Science       Date:  1984-07-27       Impact factor: 47.728

6.  Purification and structural studies of a major scrapie prion protein.

Authors:  S B Prusiner; D F Groth; D C Bolton; S B Kent; L E Hood
Journal:  Cell       Date:  1984-08       Impact factor: 41.582

7.  Scrapie prions aggregate to form amyloid-like birefringent rods.

Authors:  S B Prusiner; M P McKinley; K A Bowman; D C Bolton; P E Bendheim; D F Groth; G G Glenner
Journal:  Cell       Date:  1983-12       Impact factor: 41.582

8.  Comparison of RNA from healthy and scrapie-infected hamster brain.

Authors:  T L German; B C McMillan; B E Castle; C Dees; W F Wade; R F Marsh
Journal:  J Gen Virol       Date:  1985-04       Impact factor: 3.891

9.  Linkage of prion protein and scrapie incubation time genes.

Authors:  G A Carlson; D T Kingsbury; P A Goodman; S Coleman; S T Marshall; S DeArmond; D Westaway; S B Prusiner
Journal:  Cell       Date:  1986-08-15       Impact factor: 41.582

10.  Glycosyl-sn-1,2-dimyristylphosphatidylinositol is covalently linked to Trypanosoma brucei variant surface glycoprotein.

Authors:  M A Ferguson; M G Low; G A Cross
Journal:  J Biol Chem       Date:  1985-11-25       Impact factor: 5.157
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  16 in total

1.  PrP amyloid plaques in Creutzfeldt-Jakob disease of short duration: immunohistochemical studies of 5 cases from Poland.

Authors:  P P Liberski; H Kwiecinski; M Barcikowska; B Mirecka; J Kulczycki; E Kida; P Brown; D C Gajdusek
Journal:  Eur J Epidemiol       Date:  1991-09       Impact factor: 8.082

2.  Spontaneous assembly of a self-complementary oligopeptide to form a stable macroscopic membrane.

Authors:  S Zhang; T Holmes; C Lockshin; A Rich
Journal:  Proc Natl Acad Sci U S A       Date:  1993-04-15       Impact factor: 11.205

3.  Disruption of prion rods generates 10-nm spherical particles having high alpha-helical content and lacking scrapie infectivity.

Authors:  D Riesner; K Kellings; K Post; H Wille; H Serban; D Groth; M A Baldwin; S B Prusiner
Journal:  J Virol       Date:  1996-03       Impact factor: 5.103

4.  The most infectious prion protein particles.

Authors:  Jay R Silveira; Gregory J Raymond; Andrew G Hughson; Richard E Race; Valerie L Sim; Stanley F Hayes; Byron Caughey
Journal:  Nature       Date:  2005-09-08       Impact factor: 49.962

Review 5.  Prions, beta-sheets and transmissible dementias: is there still something missing?

Authors:  P P Liberski
Journal:  Acta Neuropathol       Date:  1995       Impact factor: 17.088

6.  Attempts to restore scrapie prion infectivity after exposure to protein denaturants.

Authors:  S B Prusiner; D Groth; A Serban; N Stahl; R Gabizon
Journal:  Proc Natl Acad Sci U S A       Date:  1993-04-01       Impact factor: 11.205

7.  Concentration and distribution of infectivity and PrPSc following partial denaturation of a mouse-adapted and a hamster-adapted scrapie strain.

Authors:  R Rubenstein; R I Carp; W Ju; C Scalici; M Papini; A Rubenstein; R Kascsak
Journal:  Arch Virol       Date:  1994       Impact factor: 2.574

8.  Molecular location of a species-specific epitope on the hamster scrapie agent protein.

Authors:  D C Bolton; S J Seligman; G Bablanian; D Windsor; L J Scala; K S Kim; C M Chen; R J Kascsak; P E Bendheim
Journal:  J Virol       Date:  1991-07       Impact factor: 5.103

9.  Scrapie infectivity correlates with converting activity, protease resistance, and aggregation of scrapie-associated prion protein in guanidine denaturation studies.

Authors:  B Caughey; G J Raymond; D A Kocisko; P T Lansbury
Journal:  J Virol       Date:  1997-05       Impact factor: 5.103

10.  Viral particles are required for infection in neurodegenerative Creutzfeldt-Jakob disease.

Authors:  L Manuelidis; T Sklaviadis; A Akowitz; W Fritch
Journal:  Proc Natl Acad Sci U S A       Date:  1995-05-23       Impact factor: 11.205
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