Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Application of ELISA for assessment of antiserum immunoreactivity in endocrine immunocytochemical studies.

Literature DB >> 6131910

Application of ELISA for assessment of antiserum immunoreactivity in endocrine immunocytochemical studies.

Abstract

An indirect method of an enzyme-linked immunosorbent assay (ELISA) is described to assess the reactivity of antisera used for the identification of peptide hormone producing cells by immunocytochemistry. Compared with radioimmunoassay and immunodiffusion, the ELISA method has the advantages of simplicity and sensitivity and represents with the hormone adsorbed to a matrix a situation more or less comparable to that in tissue sections. It is concluded that specificity testing of antisera applied in endocrine immunocytochemical studies can best be achieved by application of the ELISA method in combination with appropriate tissue controls.

Mesh：

Substances：

Year: 1983 PMID： 6131910 PMCID： PMC498235 DOI： 10.1136/jcp.36.4.406

Source DB: PubMed Journal: J Clin Pathol ISSN： 0021-9746 Impact factor: 3.411

9 in total

Review 1. Immunoperoxidase technique in histopathology: applications, methods, and controls.

Authors: E Heyderman
Journal: J Clin Pathol Date: 1979-10 Impact factor: 3.411

2. Can specificity ever be proved in immunocytochemical staining.

Authors: D F Swaab; C W Pool; F W Van Leeuwen
Journal: J Histochem Cytochem Date: 1977-05 Impact factor: 2.479

3. Detection of antigens and antibodies by an immuno-peroxidase method applied on thin longitudinal sections of SDS-polyacrylamide gels.

Authors: W van Raamsdonk; C W Pool; C Heyting
Journal: J Immunol Methods Date: 1977 Impact factor: 2.303

4. Corticotropin-releasing factor immunoreactivity in human gastrointestinal tract.

Authors: A C Nieuwenhuijzen Kruseman; E A Linton; P J Lowry; L H Rees; G M Besser
Journal: Lancet Date: 1982-12-04 Impact factor: 79.321

Review 5. Enzyme immunoassays with special reference to ELISA techniques.

Authors: A Voller; A Bartlett; D E Bidwell
Journal: J Clin Pathol Date: 1978-06 Impact factor: 3.411

6. Large-scale preparation of highly purified pyrogen-free human growth hormone for clinical use.

Authors: R L Jones; G Benker; P R Salacinski; T J Lloyd; P J Lowry
Journal: J Endocrinol Date: 1979-07 Impact factor: 4.286

7. Reliability of the enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of Trichinella spiralis infections in conventionally raised pigs.

Authors: E J Ruitenberg; P A Steerenberg; B J Brosi; J Buys
Journal: J Immunol Methods Date: 1976 Impact factor: 2.303

8. Hydrophobic-interaction chromatography and anion-exchange chromatography in the presence of acetonitrile. A two-step purification method for human prolactin.

Authors: S C Hodgkinson; P J Lowry
Journal: Biochem J Date: 1981-12-01 Impact factor: 3.857

9. Adrenocorticotrophic and melanocyte-stimulating peptides in the human pituitary.

Authors: A P Scott; P J Lowry
Journal: Biochem J Date: 1974-06 Impact factor: 3.857