Literature DB >> 6129055

Dissociation and characterization of pilin isolated from Pseudomonas aeruginosa strains PAK and PAO.

T H Watts, C M Kay, W Paranchych.   

Abstract

Pili isolated from Pseudomonas aeruginosa strains PAK and PAO have been dissociated into subunits using the detergent octyl glucoside. Circular dichroism studies indicated that no change in protein conformation occurs as a result of this treatment. Ultracentrifugation measurements showed that both pilins have a Stokes radius of 21 A (1 A = 0.1 nm) corresponding to an axial ratio of between 3:1 and 5:1, when approximated as prolate or oblate ellipsoids. Sedimentation equilibrium measurements show that even at low protein concentrations the pilin-detergent complex exists as a mixture of monomers and dimers.

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Year:  1982        PMID： 6129055     DOI： 10.1139/o82-110

Source DB:  PubMed          Journal:  Can J Biochem        ISSN： 0008-4018


  13 in total

1.  Role of peptide hydrophobicity in the mechanism of action of alpha-helical antimicrobial peptides.

Authors:  Yuxin Chen; Michael T Guarnieri; Adriana I Vasil; Michael L Vasil; Colin T Mant; Robert S Hodges
Journal:  Antimicrob Agents Chemother       Date:  2006-12-11       Impact factor: 5.191

2.  Effects of net charge and the number of positively charged residues on the biological activity of amphipathic alpha-helical cationic antimicrobial peptides.

Authors:  Ziqing Jiang; Adriana I Vasil; John D Hale; Robert E W Hancock; Michael L Vasil; Robert S Hodges
Journal:  Biopolymers       Date:  2008       Impact factor: 2.505

3.  Cross-reactive and strain-specific antipeptide antibodies to Pseudomonas aeruginosa PAK and PAO pili.

Authors:  K K Lee; W Paranchych; R S Hodges
Journal:  Infect Immun       Date:  1990-09       Impact factor: 3.441

4.  Gonococcal pili. Primary structure and receptor binding domain.

Authors:  G K Schoolnik; R Fernandez; J Y Tai; J Rothbard; E C Gotschlich
Journal:  J Exp Med       Date:  1984-05-01       Impact factor: 14.307

5.  Formation of 9-nm filaments from pilin monomers obtained by octyl-glucoside dissociation of Pseudomonas aeruginosa pili.

Authors:  T H Watts; D G Scraba; W Paranchych
Journal:  J Bacteriol       Date:  1982-09       Impact factor: 3.490

6.  An analysis of the organization and evolution of type 4 fimbrial (MePhe) subunit proteins.

Authors:  B Dalrymple; J S Mattick
Journal:  J Mol Evol       Date:  1987       Impact factor: 2.395

7.  Rational design of α-helical antimicrobial peptides to target Gram-negative pathogens, Acinetobacter baumannii and Pseudomonas aeruginosa: utilization of charge, 'specificity determinants,' total hydrophobicity, hydrophobe type and location as design parameters to improve the therapeutic ratio.

Authors:  Ziqing Jiang; Adriana I Vasil; Lajos Gera; Michael L Vasil; Robert S Hodges
Journal:  Chem Biol Drug Des       Date:  2011-02-02       Impact factor: 2.817

8.  Identification of the Pseudomonas aeruginosa 1244 pilin glycosylation site.

Authors:  Jason E Comer; Mark A Marshall; Vincent J Blanch; Carolyn D Deal; Peter Castric
Journal:  Infect Immun       Date:  2002-06       Impact factor: 3.441

9.  Mapping of the antigenic determinants of Pseudomonas aeruginosa PAK polar pili.

Authors:  T H Watts; P A Sastry; R S Hodges; W Paranchych
Journal:  Infect Immun       Date:  1983-10       Impact factor: 3.441

10.  Electrophoretic separation and molecular weight characterization of Pseudomonas aeruginosa H-antigen flagellins.

Authors:  J S Allison; M Dawson; D Drake; T C Montie
Journal:  Infect Immun       Date:  1985-09       Impact factor: 3.441
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