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Literature DB >> 6127281

Isolation of a cloned DNA segment containing a ribosomal protein gene of Drosophila melanogaster.

Abstract

A Drosophila genomic DNA library in the vector Charon 4 was screened using cDNA derived from the small (6S-12S) poly(A)+ mRNA of 2-6-h-old Drosophila embryos. This fraction of mRNA is enriched for ribosomal protein-coding sequences. The selected recombinants were hybridized to total mRNA under conditions which allowed for isolation of homologous mRNAs. The mRNA from these RNA/DNA hybrids was eluted and translated in vitro. The translation products were analyzed by one- and two-dimensional electrophoresis with authentic ribosomal proteins as standards. One cloned DNA segment was found to contain a ribosomal protein gene, and a sequence which hybridizes strongly to at least 5 other ribosomal protein mRNAs.

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Year: 1982 PMID： 6127281 DOI： 10.1016/0378-1119(82)90165-2

Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688

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Cited

9 in total

9. Sequences coding for the ribosomal protein L14 in Xenopus laevis and Xenopus tropicalis; homologies in the 5' untranslated region are shared with other r-protein mRNAs.

Authors: E Beccari; P Mazzetti; A Mileo; I Bozzoni; P Pierandrei-Amaldi; F Amaldi
Journal: Nucleic Acids Res Date: 1986-10-10 Impact factor: 16.971

9 in total

Isolation of a cloned DNA segment containing a ribosomal protein gene of Drosophila melanogaster.

1. Characterization of developing oat seed mRNA: evidence for many globulin mRNAs.

2. A Drosophila ribosomal protein gene is located near repeated sequences including rDNA sequences.

3. A new copia-like transposable element found in a Drosophila rDNA gene unit.

4. Primary structure of human ribosomal protein S14 and the gene that encodes it.

5. Isolation and characterization of cloned DNA sequences containing ribosomal protein genes of Drosophila melanogaster.

6. Sequence, structure, and codon preference of the Drosophila ribosomal protein 49 gene.

7. Isolation and structural analysis of a ribosomal protein gene in D.melanogaster.

8. Two genes for ribosomal protein 51 of Saccharomyces cerevisiae complement and contribute to the ribosomes.

9. Sequences coding for the ribosomal protein L14 in Xenopus laevis and Xenopus tropicalis; homologies in the 5' untranslated region are shared with other r-protein mRNAs.