| Literature DB >> 6099400 |
M G Koziel, T L Adams, M A Hazlet, D Damm, J Miller, D Dahlbeck, S Jayne, B J Staskawicz.
Abstract
The promoter region of the CaMV inclusion body protein gene was modified for use in chimeric gene fusions. The modified promoter was used to construct a selectable marker for plant transformation based on the Tn 5 kanamycin resistance gene. This chimeric selectable marker was introduced into plant cells using oncogenic and deoncogenized strains of Agrobacterium tumefaciens. Both types of transformation produced kanamycin-resistant cell lines. The resistant cell lines derived from the deoncogenized strains were used to regenerate shoots. A second type of selection based on the ability of octopine synthase to detoxify aminoethyl cysteine was also used to select transformants in both oncogenic and nononcogenic transformation.Entities:
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Year: 1984 PMID： 6099400
Source DB: PubMed Journal: J Mol Appl Genet ISSN： 0271-6801