Literature DB >> 3016666

Expression in plants of two bacterial antibiotic resistance genes after protoplast transformation with a new plant expression vector.

M Pietrzak, R D Shillito, T Hohn, I Potrykus.   

Abstract

Two bacterial antibiotic resistance genes, one coding for the neomycin phosphotransferase (NPT I) from Tn903, and the other coding for the chloramphenicol acetyltransferase from Tn9 were used as plant selectable markers. Both genes were introduced into the Nicotiana tabacum genome in a new plant expression vector, using the direct gene transfer method. The vector pDH51, used in these experiments contains a plant expression unit as a movable cassette, consisting of the strong cauliflower mosaic virus (CaMV) 35S RNA promoter and transcription terminator separated by a polylinker containing several unique restriction sites.

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Year:  1986        PMID： 3016666      PMCID： PMC311596          DOI： 10.1093/nar/14.14.5857

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  16 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Nucleotide sequence analysis of the chloramphenicol resistance transposon Tn9.

Authors:  N K Alton; D Vapnek
Journal:  Nature       Date:  1979 Dec 20-27       Impact factor: 49.962

3.  Streptomycin-resistant plants from callus culture of haploid tobacco.

Authors:  P Maliga; A Sz-Breznovits; L Márton
Journal:  Nat New Biol       Date:  1973-07-04

4.  Nucleotide sequence of the kanamycin resistance transposon Tn903.

Authors:  A Oka; H Sugisaki; M Takanami
Journal:  J Mol Biol       Date:  1981-04-05       Impact factor: 5.469

5.  Nucleotide sequence of the Streptococcus faecalis plasmid gene encoding the 3'5"-aminoglycoside phosphotransferase type III.

Authors:  P Trieu-Cuot; P Courvalin
Journal:  Gene       Date:  1983-09       Impact factor: 3.688

6.  Nucleotide sequence of cauliflower mosaic virus DNA.

Authors:  A Franck; H Guilley; G Jonard; K Richards; L Hirth
Journal:  Cell       Date:  1980-08       Impact factor: 41.582

Review 7.  T-DNA of the Agrobacterium Ti and Ri plasmids.

Authors:  M W Bevan; M D Chilton
Journal:  Annu Rev Genet       Date:  1982       Impact factor: 16.830

8.  Restriction map of native and cloned cauliflower mosaic virus DNA.

Authors:  T Hohn; B Hohn; A Lesot; G Lebeurier
Journal:  Gene       Date:  1980-10       Impact factor: 3.688

9.  Aphid transmission and a polypeptide are specified by a defined region of the cauliflower mosaic virus genome.

Authors:  C J Woolston; S N Covey; J R Penswick; J W Davies
Journal:  Gene       Date:  1983-07       Impact factor: 3.688

10.  Mutagenesis of cauliflower mosaic virus.

Authors:  L K Dixon; I Koenig; T Hohn
Journal:  Gene       Date:  1983-11       Impact factor: 3.688
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  134 in total

1.  Termination and peptide release at the upstream open reading frame are required for downstream translation on synthetic shunt-competent mRNA leaders.

Authors:  M Hemmings-Mieszczak; T Hohn; T Preiss
Journal:  Mol Cell Biol       Date:  2000-09       Impact factor: 4.272

2.  Early and multiple Ac transpositions in rice suitable for efficient insertional mutagenesis.

Authors:  R Greco; P B Ouwerkerk; A J Taal; C Favalli; T Beguiristain; P Puigdomènech; L Colombo; J H Hoge; A Pereira
Journal:  Plant Mol Biol       Date:  2001-05       Impact factor: 4.076

3.  Wind-induced plant motion immediately increases cytosolic calcium.

Authors:  M R Knight; S M Smith; A J Trewavas
Journal:  Proc Natl Acad Sci U S A       Date:  1992-06-01       Impact factor: 11.205

4.  A test for transvection in plants: DNA pairing may lead to trans-activation or silencing of complex heteroalleles in tobacco.

Authors:  M Matzke; M F Mette; J Jakowitsch; T Kanno; E A Moscone; J van der Winden; A J Matzke
Journal:  Genetics       Date:  2001-05       Impact factor: 4.562

5.  Transpositional behaviour of an Ac/Ds system for reverse genetics in rice.

Authors:  R Greco; P B F Ouwerkerk; R J De Kam; C Sallaud; C Favalli; L Colombo; E Guiderdoni; A H Meijer; J H C Hoge Dagger; A Pereira
Journal:  Theor Appl Genet       Date:  2003-09-25       Impact factor: 5.699

6.  Subcellular location and expression level of a chimeric protein consisting of the maize waxy transit peptide and the beta-glucuronidase of Escherichia coli in transgenic potato plants.

Authors:  R B Klösgen; J H Weil
Journal:  Mol Gen Genet       Date:  1991-02

7.  Stable transformation of the moss Physcomitrella patens.

Authors:  D Schaefer; J P Zryd; C D Knight; D J Cove
Journal:  Mol Gen Genet       Date:  1991-05

8.  Agrobacterium-Mediated Transformation of Subterranean Clover (Trifolium subterraneum L.).

Authors:  MRI. Khan; L. M. Tabe; L. C. Heath; D. Spencer; TJV. Higgins
Journal:  Plant Physiol       Date:  1994-05       Impact factor: 8.340

9.  Repression of formate dehydrogenase in Solanum tuberosum increases steady-state levels of formate and accelerates the accumulation of proline in response to osmotic stress.

Authors:  Françoise Ambard-Bretteville; Céline Sorin; Fabrice Rébeillé; Cécile Hourton-Cabassa; Catherine Colas des Francs-Small
Journal:  Plant Mol Biol       Date:  2003-08       Impact factor: 4.076

10.  Cauliflower mosaic virus promoters direct efficient expression of a bacterial G418 resistance gene in Schizosaccharomyces pombe.

Authors:  H Gmünder; J Kohli
Journal:  Mol Gen Genet       Date:  1989-12
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