Literature DB >> 6098808

Growth conditions of F9 embryonal carcinoma cells affect the degree of DNA methylation.

R P Erickson, S Ferrucci, B Rahe, M P Rosenberg, D Morello.   

Abstract

We have investigated differences in C pG methylation between F9 embryonal carcinoma cells in vitro and as tumor cells grown in vivo using Msp I and Hpa II restriction isoschizomers. Southerns were hybridized with two low copy number probes, mouse major beta-globin (f7) and a class I, histocompatibility-2 cDNA clone (pH-2d-4). In each case, the tumor-DNA was hypomethylated while the DNA from F9 cells grown in vitro was moderately methylated. We conclude that growth conditions or cell-cell interactions can greatly affect methylation of C pG sites.

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Year:  1984        PMID： 6098808     DOI： 10.1007/bf00776983

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  33 in total

1.  The induction of differentiation in teratocarcinoma stem cells by retinoic acid.

Authors:  S Strickland; V Mahdavi
Journal:  Cell       Date:  1978-10       Impact factor: 41.582

2.  Comparison of nucleotide sequences of mRNAs belonging to the mouse H-2 multigene family.

Authors:  J L Lalanne; F Bregegere; C Delarbre; J P Abastado; G Gachelin; P Kourilsky
Journal:  Nucleic Acids Res       Date:  1982-02-11       Impact factor: 16.971

3.  DNA sequence organization of the beta-globin complex in the BALB/c mouse.

Authors:  C L Jahn; C A Hutchison; S J Phillips; S Weaver; N L Haigwood; C F Voliva; M H Edgell
Journal:  Cell       Date:  1980-08       Impact factor: 41.582

4.  Immunoglobulin heavy chain genes: demethylation accompanies class switching.

Authors:  J Rogers; R Wall
Journal:  Proc Natl Acad Sci U S A       Date:  1981-12       Impact factor: 11.205

5.  Temperature-sensitive changes in the structure of globin chromatin in lines of red cell precursors transformed by ts-AEV.

Authors:  H Weintraub; H Beug; M Groudine; T Graf
Journal:  Cell       Date:  1982-04       Impact factor: 41.582

6.  Tissue-specific DNA methylation in a cluster of rabbit beta-like globin genes.

Authors:  C K Shen; T Maniatis
Journal:  Proc Natl Acad Sci U S A       Date:  1980-11       Impact factor: 11.205

7.  The genetics of teratocarcinoma transplantation: tumor formation in allogeneic hosts by the embryonal carcinoma cell lines F9 and PCC3.

Authors:  P R Avner; W F Dove; P Dubois; J A Gaillard; J L Guénet; F Jacob; H Jakob; A Shedlovsky
Journal:  Immunogenetics       Date:  1978-12       Impact factor: 2.846

8.  Replication and expression of Xenopus laevis globin genes injected into fertilized Xenopus eggs.

Authors:  M M Bendig; J G Williams
Journal:  Proc Natl Acad Sci U S A       Date:  1983-10       Impact factor: 11.205

9.  Surface antigens common to mouse cleavage embryos and primitive teratocarcinoma cells in culture.

Authors:  K Artzt; P Dubois; D Bennett; H Condamine; C Babinet; F Jacob
Journal:  Proc Natl Acad Sci U S A       Date:  1973-10       Impact factor: 11.205

10.  Molecular mechanisms of human hemoglobin switching: selective undermethylation and expression of globin genes in embryonic, fetal, and adult erythroblasts.

Authors:  F Mavilio; A Giampaolo; A Carè; G Migliaccio; M Calandrini; G Russo; G L Pagliardi; G Mastroberardino; M Marinucci; C Peschle
Journal:  Proc Natl Acad Sci U S A       Date:  1983-11       Impact factor: 11.205
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