Literature DB >> 6095270

Construction of a retrovirus capable of transducing and expressing genes in multipotential embryonic cells.

J L Rubenstein, J F Nicolas, F Jacob.   

Abstract

Retroviral gene expression is inhibited in embryonal carcinoma (EC) cells. We have constructed a recombinant retroviral vector that is capable of expressing the neomycin-resistance (neo) gene in EC cells. The critical modification that permits expression of the neo gene is the insertion of a composite simian virus 40 early gene-herpes simplex virus type 1 thymidine kinase gene (SVtk) promoter 3' to the viral first intron and 5' to the neo gene. When the SVtk promoter is deleted, the recombinant retrovirus is either unable or extremely inefficient at expressing the neo gene in EC cells.

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Year:  1984        PMID： 6095270      PMCID： PMC392092          DOI： 10.1073/pnas.81.22.7137

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  27 in total

1.  [Mouse teratocarcinoma: differentiation in cultures of a multipotential primitive cell line (author's transl)].

Authors:  J F Nicolas; P Dubois; H Jakob; J Gaillard; F Jacob
Journal:  Ann Microbiol (Paris)       Date:  1975-01

2.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

3.  A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors:  F L Graham; A J van der Eb
Journal:  Virology       Date:  1973-04       Impact factor: 3.616

4.  Nucleotide sequence of Moloney murine leukaemia virus.

Authors:  T M Shinnick; R A Lerner; J G Sutcliffe
Journal:  Nature       Date:  1981 Oct 15-21       Impact factor: 49.962

5.  Formation of infectious progeny virus after insertion of herpes simplex thymidine kinase gene into DNA of an avian retrovirus.

Authors:  K Shimotohno; H M Temin
Journal:  Cell       Date:  1981-10       Impact factor: 41.582

6.  Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.

Authors:  P J Southern; P Berg
Journal:  J Mol Appl Genet       Date:  1982

7.  DNA methylation and gene expression: endogenous retroviral genome becomes infectious after molecular cloning.

Authors:  K Harbers; A Schnieke; H Stuhlmann; D Jähner; R Jaenisch
Journal:  Proc Natl Acad Sci U S A       Date:  1981-12       Impact factor: 11.205

8.  Infection of preimplantation mouse embryos and of newborn mice with leukemia virus: tissue distribution of viral DNA and RNA and leukemogenesis in the adult animal.

Authors:  R Jaenisch; H Fan; B Croker
Journal:  Proc Natl Acad Sci U S A       Date:  1975-10       Impact factor: 11.205

9.  Measurement of the sequence complexity of cloned Moloney murine leukemia virus 60 to 70S RNA: evidence for a haploid genome.

Authors:  H Fan; M Paskind
Journal:  J Virol       Date:  1974-09       Impact factor: 5.103

10.  Chromosomal position and activation of retroviral genomes inserted into the germ line of mice.

Authors:  R Jaenisch; D Jähner; P Nobis; I Simon; J Löhler; K Harbers; D Grotkopp
Journal:  Cell       Date:  1981-05       Impact factor: 41.582
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  33 in total

1.  High-frequency intracellular transposition of a defective mammalian provirus detected by an in situ colorimetric assay.

Authors:  T Tchenio; T Heidmann
Journal:  J Virol       Date:  1992-03       Impact factor: 5.103

2.  Promoter interactions in retrovirus vectors introduced into fibroblasts and embryonic stem cells.

Authors:  P Soriano; G Friedrich; P Lawinger
Journal:  J Virol       Date:  1991-05       Impact factor: 5.103

3.  Immortalization of bipotential and plastic glio-neuronal precursor cells.

Authors:  C Evrard; I Borde; P Marin; E Galiana; J Prémont; F Gros; P Rouget
Journal:  Proc Natl Acad Sci U S A       Date:  1990-04       Impact factor: 11.205

4.  Transfer of single gene-containing long terminal repeats into the genome of mammalian cells by a retroviral vector carrying the cre gene and the loxP site.

Authors:  A Choulika; V Guyot; J F Nicolas
Journal:  J Virol       Date:  1996-03       Impact factor: 5.103

5.  Expression of human class II major histocompatibility complex antigens using retrovirus vectors.

Authors:  A J Korman; J D Frantz; J L Strominger; R C Mulligan
Journal:  Proc Natl Acad Sci U S A       Date:  1987-04       Impact factor: 11.205

6.  Retroviral vector gene expression in F9 embryonal carcinoma cells.

Authors:  E Linney; S D Neill; D S Prestridge
Journal:  J Virol       Date:  1987-10       Impact factor: 5.103

7.  An embryonic DNA-binding protein specific for the promoter of the retrovirus long terminal repeat.

Authors:  F Flamant; C C Gurin; J A Sorge
Journal:  Mol Cell Biol       Date:  1987-10       Impact factor: 4.272

8.  Quantitative analysis of gene suppression in integrated retrovirus vectors.

Authors:  M Emerman; H M Temin
Journal:  Mol Cell Biol       Date:  1986-03       Impact factor: 4.272

9.  Development of a retroviral vector for inducible expression of transforming growth factor beta 1.

Authors:  M L McGeady; P M Arthur; M Seidman
Journal:  J Virol       Date:  1990-07       Impact factor: 5.103

10.  Activator-dependent and activator-independent defective recombinant retroviruses from bovine leukemia virus.

Authors:  D Milan; J F Nicolas
Journal:  J Virol       Date:  1991-04       Impact factor: 5.103
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