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Literature DB >> 6094182

Alternative splicing in the 5' moiety of the H-2Kd gene transcript.

Abstract

The H-2Kd gene, which encodes a mouse major transplantation antigen, was transfected into L TK- mouse fibroblasts. Two transcripts of the gene were detected by S1 nuclease mapping analysis. They correspond to two previously characterized cDNA clones isolated from DBA/2 mouse liver RNA, leading to the conclusion that the H-2Kd gene gives rise to two distinct transcripts through an alternate use of splicing sites. The non-canonical RNA potentially encodes a so far undescribed H-2Kd-like molecule. It is present in all tissues tested (liver, spleen, thymus, kidney) albeit in lower amounts (approximately 10-fold less) than the canonical RNA coding for H-2Kd.

Entities: Chemical

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Year: 1984 PMID： 6094182 PMCID： PMC557697 DOI： 10.1002/j.1460-2075.1984.tb02143.x

Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598

31 in total

1. Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells.

Authors: M Wigler; S Silverstein; L S Lee; A Pellicer; Y c Cheng; R Axel
Journal: Cell Date: 1977-05 Impact factor: 41.582

2. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.

Authors: A J Berk; P A Sharp
Journal: Cell Date: 1977-11 Impact factor: 41.582

3. Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping.

Authors: J Favaloro; R Treisman; R Kamen
Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

4. Further molecular complexities of H-2 K- and D-region antigens.

Authors: P Démant; D Iványi
Journal: Nature Date: 1981-03-12 Impact factor: 49.962

5. Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors: A M Maxam; W Gilbert
Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

6. Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12.

Authors: F Colbere-Garapin; S Chousterman; F Horodniceanu; P Kourilsky; A C Garapin
Journal: Proc Natl Acad Sci U S A Date: 1979-08 Impact factor: 11.205

7. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression.

Authors: K Ozato; D H Sachs
Journal: J Immunol Date: 1981-01 Impact factor: 5.422

8. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.

Authors: C Auffray; F Rougeon
Journal: Eur J Biochem Date: 1980-06

9. Mouse histocompatibility genes: structure and organisation of a Kd gene.

Authors: S Kvist; L Roberts; B Dobberstein
Journal: EMBO J Date: 1983 Impact factor: 11.598

10. The DNA sequence of the H-2kb gene: evidence for gene conversion as a mechanism for the generation of polymorphism in histocompatibilty antigens.

Authors: E Weiss; L Golden; R Zakut; A Mellor; K Fahrner; S Kvist; R A Flavell
Journal: EMBO J Date: 1983 Impact factor: 11.598

15 in total

10. Primary structure of the gene for the murine Ia antigen-associated invariant chains (Ii). An alternatively spliced exon encodes a cysteine-rich domain highly homologous to a repetitive sequence of thyroglobulin.

Authors: N Koch; W Lauer; J Habicht; B Dobberstein
Journal: EMBO J Date: 1987-06 Impact factor: 11.598

Alternative splicing in the 5' moiety of the H-2Kd gene transcript.

1. Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells.

2. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.

3. Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping.

4. Further molecular complexities of H-2 K- and D-region antigens.

5. Sequencing end-labeled DNA with base-specific chemical cleavages.

6. Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12.

7. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression.

8. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.

9. Mouse histocompatibility genes: structure and organisation of a Kd gene.

10. The DNA sequence of the H-2kb gene: evidence for gene conversion as a mechanism for the generation of polymorphism in histocompatibilty antigens.

1. Transmembrane and cytoplasmic domain sequences demonstrate at least two expressed bovine MHC class I loci.

2. Isolation, expression, and the primary structure of HLA-Cw1 and HLA-Cw2 genes: evolutionary aspects.

3. Structure and polymorphism of class I MHC antigen mRNA.

Review 4. Multiple class I molecules generated from single genes by alternative splicing of pre-mRNAs.

5. Alternative protein products with different carboxyl termini from a single class I gene, H-2Kb.

6. Alternative splicing in the mouse H-2Kd gene is not necessary for the classical Kd antigen function.

7. Three new class I HLA alleles: structure of mRNAs and alternative mechanisms of processing.

8. An H-2K gene of the tw32 mutant at the T/t complex is a close parent of an H-2Kq gene.

9. Asynchronous regulation of mouse H-2D and beta-2 microglobulin RNA transcripts.

10. Primary structure of the gene for the murine Ia antigen-associated invariant chains (Ii). An alternatively spliced exon encodes a cysteine-rich domain highly homologous to a repetitive sequence of thyroglobulin.