Literature DB >> 226978

Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12.

F Colbere-Garapin, S Chousterman, F Horodniceanu, P Kourilsky, A C Garapin.   

Abstract

A herpes simplex virus DNA fragment that is produced by digestion with BamHI endonuclease and carries the thymidine kinase (TK; ATP:thymidine 5'-phosphotransferase, EC 2.7.1.21) gene has been cloned in Escherichia coli. A recombinat plasmid, pFG5, has been analyzed extensively and a detailed restriction map is presented. pFG5 DNA efficiently transforms TK- mouse L cells. The TK coding sequence in the cloned fragment has been localized and a smaller recombinant plasmid, pAG0, also carrying an active TK gene, has been constructed to serve as a more convenient vector for transfer, into TK- cells, of genes previously cloned in E. coli.

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Year:  1979        PMID： 226978      PMCID： PMC383912          DOI： 10.1073/pnas.76.8.3755

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  22 in total

1.  Enhanced plating efficiency of herpesvirus DNA on herpes simplex virus type 1 DNA-transformed cells.

Authors:  F Colbère-Garapin; F Horodniceanu
Journal:  J Natl Cancer Inst       Date:  1979-01       Impact factor: 13.506

2.  Transfer of the gene for thymidine kinase to thymidine kinase-deficient human cells by purified herpes simplex viral DNA.

Authors:  S Bacchetti; F L Graham
Journal:  Proc Natl Acad Sci U S A       Date:  1977-04       Impact factor: 11.205

3.  Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells.

Authors:  M Wigler; S Silverstein; L S Lee; A Pellicer; Y c Cheng; R Axel
Journal:  Cell       Date:  1977-05       Impact factor: 41.582

4.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

5.  The transfer and stable integration of the HSV thymidine kinase gene into mouse cells.

Authors:  A Pellicer; M Wigler; R Axel; S Silverstein
Journal:  Cell       Date:  1978-05       Impact factor: 41.582

6.  Introduction of the herpes simplex virus thymidine kinase gene into mouse cells using virus DNA or transformed cell DNA.

Authors:  A C Minson; P Wildy; A Buchan; G Darby
Journal:  Cell       Date:  1978-03       Impact factor: 41.582

7.  Characterization of the mRNA for herpes simplex virus thymidine kinase by cell-free synthesis of active enzyme.

Authors:  K Cremer; M Bodemer; W C Summers
Journal:  Nucleic Acids Res       Date:  1978-07       Impact factor: 16.971

8.  Cloning of herpes simplex type 1 DNA fragments in a bacteriophage lambda vector.

Authors:  L W Enquist; M J Madden; P Schiop-Stanley; G F Vande Woude
Journal:  Science       Date:  1979-02-09       Impact factor: 47.728

9.  Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor.

Authors:  M Wigler; A Pellicer; S Silverstein; R Axel
Journal:  Cell       Date:  1978-07       Impact factor: 41.582

10.  An improved technique for obtaining enhanced infectivity with herpes simplex virus type 1 DNA.

Authors:  N D Stow; N M Wilkie
Journal:  J Gen Virol       Date:  1976-12       Impact factor: 3.891
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  109 in total

1.  In vitro immortalization of marmoset cells with three subgroups of herpesvirus saimiri.

Authors:  E Szomolanyi; P Medveczky; C Mulder
Journal:  J Virol       Date:  1987-11       Impact factor: 5.103

2.  The Epstein-Barr virus BNLF-1 membrane protein LMP1 induces homotypic adhesion mediated by CD11a/CD18 in a murine B-cell line, mimicking the action of phorbol ester.

Authors:  R Salcedo; S M Fuerstenberg; M Patarroyo; G Winberg
Journal:  J Virol       Date:  1991-10       Impact factor: 5.103

3.  Structural and functional analysis of a polyoma-related mammalian plasmid (L factor): the enhancer activity and plasmid establishment.

Authors:  H Yoshimura; Y Ikeda; M Yoshimoto; S Tamaki; K Hanada; T Kusano; T Kohda; H Saito; M Oishi
Journal:  Nucleic Acids Res       Date:  1991-07-11       Impact factor: 16.971

4.  A dominant positive and negative selectable gene for use in mammalian cells.

Authors:  F Schwartz; N Maeda; O Smithies; R Hickey; W Edelmann; A Skoultchi; R Kucherlapati
Journal:  Proc Natl Acad Sci U S A       Date:  1991-12-01       Impact factor: 11.205

5.  Modulation of tk expression in mouse pericentromeric heterochromatin.

Authors:  K Butner; C W Lo
Journal:  Mol Cell Biol       Date:  1986-12       Impact factor: 4.272

6.  Construction of new yeast vectors and cloning of the nif (nitrogen fixation) gene cluster of Klebsiella pneumoniae in yeast.

Authors:  C Gerbaud; C Elmerich; N Tandeau de Marsac; P Chocat; N Charpin; M Guérineau; J P Aubert
Journal:  Curr Genet       Date:  1981-07       Impact factor: 3.886

7.  Introduction of hereditary disease-associated mutations into the beta-amyloid precursor protein gene of mouse embryonic stem cells: a comparison of homologous recombination methods.

Authors:  M Gschwind; G Huber
Journal:  Mol Cell Biol       Date:  1998-08       Impact factor: 4.272

8.  Ganciclovir mediated regression of rat brain tumors expressing the herpes simplex virus thymidine kinase imaged by magnetic resonance.

Authors:  A Maron; T Gustin; I Mottet; R Demeure; J N Octave
Journal:  J Neurooncol       Date:  1995       Impact factor: 4.130

9.  Efficient expression of tum- antigen P91A by transfected subgenic fragments.

Authors:  P Chomez; E De Plaen; A Van Pel; C De Smet; J P Szikora; C Lurquin; A M Lebacq-Verheyden; T Boon
Journal:  Immunogenetics       Date:  1992       Impact factor: 2.846

10.  Control of Herpes simplex virus thymidine kinase gene expression in Saccharomyces cerevisiae by a yeast promoter sequence.

Authors:  X L Zhu; C Ward; A Weissbach
Journal:  Mol Gen Genet       Date:  1984
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