Literature DB >> 6093629

Determination of diadenosine 5',5''',-P1,P4-tetraphosphate levels in cultured mammalian cells.

J C Baker, M K Jacobson.   

Abstract

A simple method for measuring the cellular content of diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) in cultured mammalian cells is described. Ap4A was rapidly extracted by dissolving cell monolayers using 0.1 N NaOH. It was separated from the bulk of cellular components in a single step by selective adsorption to a highly specific boronate affinity resin. Subpicomole amounts were quantified by a luciferin-luciferase bioluminescence assay performed in the presence of alkaline phosphatase and venom phosphodiesterase. The selectivity of this assay for Ap4A in cultured mouse cells was established by high-performance liquid chromatography. This method allows the routine measurement of subpicomole amounts of Ap4A derived from a single dish of cells.

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Year:  1984        PMID： 6093629     DOI： 10.1016/0003-2697(84)90070-8

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  13 in total

1.  An apaH mutation causes AppppA to accumulate and affects motility and catabolite repression in Escherichia coli.

Authors:  S B Farr; D N Arnosti; M J Chamberlin; B N Ames
Journal:  Proc Natl Acad Sci U S A       Date:  1989-07       Impact factor: 11.205

2.  Isolation, characterization, and inactivation of the APA1 gene encoding yeast diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase.

Authors:  P Plateau; M Fromant; J M Schmitter; J M Buhler; S Blanquet
Journal:  J Bacteriol       Date:  1989-12       Impact factor: 3.490

3.  Catabolism of bis(5'-nucleosidyl) tetraphosphates in Saccharomyces cerevisiae.

Authors:  P Plateau; M Fromant; J M Schmitter; S Blanquet
Journal:  J Bacteriol       Date:  1990-12       Impact factor: 3.490

4.  In vivo synthesis of adenylylated bis(5'-nucleosidyl) tetraphosphates (Ap4N) by Escherichia coli aminoacyl-tRNA synthetases.

Authors:  A Brevet; J Chen; F Lévêque; P Plateau; S Blanquet
Journal:  Proc Natl Acad Sci U S A       Date:  1989-11       Impact factor: 11.205

5.  Intracellular 5',5'-dinucleoside polyphosphate levels remain constant during the Escherichia coli cell cycle.

Authors:  P Plateau; M Fromant; F Kepes; S Blanquet
Journal:  J Bacteriol       Date:  1987-01       Impact factor: 3.490

6.  In vivo levels of diadenosine tetraphosphate and adenosine tetraphospho-guanosine in Physarum polycephalum during the cell cycle and oxidative stress.

Authors:  P N Garrison; S A Mathis; L D Barnes
Journal:  Mol Cell Biol       Date:  1986-04       Impact factor: 4.272

7.  Cell cycle variations of dinucleoside polyphosphates in synchronized cultures of mammalian cells.

Authors:  G Orfanoudakis; M Baltzinger; D Meyer; N Befort; J P Ebel; J J Befort; P Remy
Journal:  Mol Cell Biol       Date:  1987-07       Impact factor: 4.272

8.  Chemical modification of a functional arginine residue in diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) phosphorylase I from Saccharomyces cerevisiae.

Authors:  A K Robinson; L D Barnes
Journal:  Biochem J       Date:  1991-10-01       Impact factor: 3.857

9.  Isolation and characterization of a dinucleoside triphosphatase from Saccharomyces cerevisiae.

Authors:  A Brevet; J Chen; M Fromant; S Blanquet; P Plateau
Journal:  J Bacteriol       Date:  1991-09       Impact factor: 3.490

10.  Near-UV stress in Salmonella typhimurium: 4-thiouridine in tRNA, ppGpp, and ApppGpp as components of an adaptive response.

Authors:  G F Kramer; J C Baker; B N Ames
Journal:  J Bacteriol       Date:  1988-05       Impact factor: 3.490
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