Behavioral sequences as an easily quantifiable parameter in experimental studies.

In behavioral pharmacology frequencies and/or durations of postures are used as easily quantifiable parameters. However, significant information is also contained in the sequential order of the different behavioral elements; structural changes in behavior are not necessarily reflected in the totals of durations and frequencies. Furthermore, observed changes in these parameters might be better understood if the change in structure is known. Presently, parameters for sequences are barely used, probably because they require larger groups of animals and more sophisticated computer programs. In this paper a procedure, using a simple set of computer programs (see Appendices), is demonstrated for transformation of behavioral sequences into transition matrices, including a procedure for creating the minimal number of transitions required for further statistical analysis.