Literature DB >> 6093051

Structural analysis of the two tandemly repeated acid phosphatase genes in yeast.

W Bajwa, B Meyhack, H Rudolph, A M Schweingruber, A Hinnen.   

Abstract

We have sequenced the genetically linked genes for repressible (PHO5) and and constitutive (PHO3) acid phosphatase from S. cerevisiae. Both genes are located on a 3.91 Kb BamHI and HpaI fragment, in the order (5') PHO5, PHO3 (3'). The mRNA transcripts have been analysed by S1-nuclease mapping. They show heterogenous initiation sites. Each of the PHO5 and PHO3 genes codes for 467 amino acids as deduced from the DNA sequence. The coding regions of the two genes show homology both at the nucleotide (82%) and the amino acid (87%) level. In the coding sequences, long stretches of homologous regions are flanked by small non-homologous regions. The nucleotide homology (65%) extends to some length into the 5' and 3' non-coding flanking sequences. Further upstream sequences are unrelated. The comparison of the NH2-terminal amino acid sequence deduced from the nucleotide sequence, with that of purified repressible acid phosphatase revealed the presence of a putative signal peptide.

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Year:  1984        PMID： 6093051      PMCID： PMC320196          DOI： 10.1093/nar/12.20.7721

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  45 in total

1.  Size of primary transcripts in Ehrlich ascites cells as measured by tetraphosphate determination.

Authors:  C D Schmincke; K Herrmann; P Hausen
Journal:  Proc Natl Acad Sci U S A       Date:  1976-06       Impact factor: 11.205

2.  Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.

Authors:  A J Berk; P A Sharp
Journal:  Cell       Date:  1977-11       Impact factor: 41.582

Review 3.  Sequence analysis of eukaryotic mRNA.

Authors:  N J Proudfoot; C C Cheng; G G Brownlee
Journal:  Prog Nucleic Acid Res Mol Biol       Date:  1976

4.  Genes coding for the structure of the acid phosphatases in Saccharomyces cerevisiae.

Authors:  A Toh-e; S Kakimoto
Journal:  Mol Gen Genet       Date:  1975-12-30

5.  The use of thin acrylamide gels for DNA sequencing.

Authors:  F Sanger; A R Coulson
Journal:  FEBS Lett       Date:  1978-03-01       Impact factor: 4.124

6.  The ovalbumin gene-sequence of putative control regions.

Authors:  C Benoist; K O'Hare; R Breathnach; P Chambon
Journal:  Nucleic Acids Res       Date:  1980-01-11       Impact factor: 16.971

7.  Structural comparison of two nontandemly repeated yeast glyceraldehyde-3-phosphate dehydrogenase genes.

Authors:  J P Holland; M J Holland
Journal:  J Biol Chem       Date:  1980-03-25       Impact factor: 5.157

8.  Isolation and sequence of the gene for iso-2-cytochrome c in Saccharomyces cerevisiae.

Authors:  D L Montgomery; D W Leung; M Smith; P Shalit; G Faye; B D Hall
Journal:  Proc Natl Acad Sci U S A       Date:  1980-01       Impact factor: 11.205

9.  The primary structure of a glyceraldehyde-3-phosphate dehydrogenase gene from Saccharomyces cerevisiae.

Authors:  J P Holland; M J Holland
Journal:  J Biol Chem       Date:  1979-10-10       Impact factor: 5.157

10.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205
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  42 in total

1.  Synthesis of a gene for human serum albumin and its expression in Saccharomyces cerevisiae.

Authors:  M Kálmán; I Cserpán; G Bajszár; A Dobi; E Horváth; C Pázmán; A Simoncsits
Journal:  Nucleic Acids Res       Date:  1990-10-25       Impact factor: 16.971

2.  The acid phosphatase genes PHO10 and PHO11 in S. cerevisiae are located at the telomeres of chromosomes VIII and I.

Authors:  U Venter; W Hörz
Journal:  Nucleic Acids Res       Date:  1989-02-25       Impact factor: 16.971

3.  Different classes of polyadenylation sites in the yeast Saccharomyces cerevisiae.

Authors:  S Irniger; C M Egli; G H Braus
Journal:  Mol Cell Biol       Date:  1991-06       Impact factor: 4.272

4.  Cloning and characterization of a cDNA encoding a maize seedling phytase.

Authors:  S Maugenest; I Martinez; A M Lescure
Journal:  Biochem J       Date:  1997-03-01       Impact factor: 3.857

5.  Evolution of a bifunctional enzyme: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.

Authors:  J F Bazan; R J Fletterick; S J Pilkis
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205

6.  A consensus transcription termination sequence in the promoter region is necessary for efficient gene expression of the TRP1 gene of Saccharomyces cerevisiae.

Authors:  G Braus; G Paravicini; R Hütter
Journal:  Mol Gen Genet       Date:  1988-06

7.  Identification of two factors which bind to the upstream sequences of a number of nuclear genes coding for mitochondrial proteins and to genetic elements important for cell division in yeast.

Authors:  J C Dorsman; W C van Heeswijk; L A Grivell
Journal:  Nucleic Acids Res       Date:  1988-08-11       Impact factor: 16.971

8.  Molecular cloning, expression and evaluation of phosphohydrolases for phytate-degrading activity.

Authors:  E Moore; V R Helly; O M Conneely; P P Ward; R F Power; D R Headon
Journal:  J Ind Microbiol       Date:  1995-05

9.  The two positively acting regulatory proteins PHO2 and PHO4 physically interact with PHO5 upstream activation regions.

Authors:  K Vogel; W Hörz; A Hinnen
Journal:  Mol Cell Biol       Date:  1989-05       Impact factor: 4.272

10.  Acid phosphatase-1(1), a tightly linked molecular marker for root-knot nematode resistance in tomato: from protein to gene, using PCR and degenerate primers containing deoxyinosine.

Authors:  J M Aarts; J G Hontelez; P Fischer; R Verkerk; A van Kammen; P Zabel
Journal:  Plant Mol Biol       Date:  1991-04       Impact factor: 4.076
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