Literature DB >> 6092925

Short-term, high-efficiency expression of transfected DNA.

D J Sussman, G Milman.   

Abstract

We have achieved high-efficiency uptake and expression of foreign DNA in mouse Ltk- cells by modifying the DEAE-dextran-mediated transfection method of McCutchan and Pagano (J. Natl. Cancer Inst. 42:351-357, 1968) to include an initial incubation at elevated pH followed by a shock treatment with dimethyl sulfoxide. Up to 80% of mouse Ltk- cells transfected with the herpes simplex virus thymidine kinase gene expressed thymidine kinase as measured by autoradiography.

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Year:  1984        PMID： 6092925      PMCID： PMC368960          DOI： 10.1128/mcb.4.8.1641-1643.1984

Source DB:  PubMed          Journal:  Mol Cell Biol        ISSN： 0270-7306            Impact factor:   4.272


  12 in total

1.  Enchancement of the infectivity of simian virus 40 deoxyribonucleic acid with diethylaminoethyl-dextran.

Authors:  J H McCutchan; J S Pagano
Journal:  J Natl Cancer Inst       Date:  1968-08       Impact factor: 13.506

2.  A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors:  F L Graham; A J van der Eb
Journal:  Virology       Date:  1973-04       Impact factor: 3.616

3.  Efficient DNA transfection and rapid assay for thymidine kinase activity and viral antigenic determinants.

Authors:  G Milman; M Herzberg
Journal:  Somatic Cell Genet       Date:  1981-03

4.  SV40 DNA transfection of cells in suspension: analysis of efficiency of transcription and translation of T-antigen.

Authors:  G Chu; P A Sharp
Journal:  Gene       Date:  1981-03       Impact factor: 3.688

5.  A better cell line for making hybridomas secreting specific antibodies.

Authors:  M Shulman; C D Wilde; G Köhler
Journal:  Nature       Date:  1978-11-16       Impact factor: 49.962

6.  Mitogenic effect of alkaline pH on quiescent, serum-starved cells.

Authors:  A Zetterberg; W Engström
Journal:  Proc Natl Acad Sci U S A       Date:  1981-07       Impact factor: 11.205

7.  Regulation of simian virus 40 transcription: sensitive analysis of the RNA species present early in infections by virus or viral DNA.

Authors:  B A Parker; G R Stark
Journal:  J Virol       Date:  1979-08       Impact factor: 5.103

8.  Co-transfer of human X-linked markers into murine somatic cells via isolated metaphase chromosomes.

Authors:  C L Miller; F H Ruddle
Journal:  Proc Natl Acad Sci U S A       Date:  1978-07       Impact factor: 11.205

9.  Efficient infection of monkey cells with DNA of simian virus 40.

Authors:  L M Sompayrac; K J Danna
Journal:  Proc Natl Acad Sci U S A       Date:  1981-12       Impact factor: 11.205

10.  Parameters governing the transfer of the genes for thymidine kinase and dihydrofolate reductase into mouse cells using metaphase chromosomes or DNA.

Authors:  W H Lewis; P R Srinivasan; N Stokoe; L Siminovitch
Journal:  Somatic Cell Genet       Date:  1980-05
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  54 in total

1.  Cystein 402 of HIV gp 120 is essential for CD4-binding and resistance of gp 120 to intracellular degradation.

Authors:  A Hemming; A Bolmstedt; P Flodby; L Lundberg; M Gidlund; H Wigzell; S Olofsson
Journal:  Arch Virol       Date:  1989       Impact factor: 2.574

2.  Topoisomerase I is preferentially associated with normal SV40 replicative intermediates, but is associated with both replicating and nonreplicating SV40 DNAs which are deficient in histones.

Authors:  J J Champoux
Journal:  Nucleic Acids Res       Date:  1992-07-11       Impact factor: 16.971

3.  Differential determinants for coupling of distinct G proteins with the class B secretin receptor.

Authors:  Gene L Garcia; Maoqing Dong; Laurence J Miller
Journal:  Am J Physiol Cell Physiol       Date:  2012-01-25       Impact factor: 4.249

4.  Transfection of HepG2 cells with infectious hepatitis C virus genome.

Authors:  S Dash; A B Halim; H Tsuji; N Hiramatsu; M A Gerber
Journal:  Am J Pathol       Date:  1997-08       Impact factor: 4.307

5.  Biological characteristics of the leukemia-associated transcriptional factor AML1 disclosed by hematopoietic rescue of AML1-deficient embryonic stem cells by using a knock-in strategy.

Authors:  T Okuda; K Takeda; Y Fujita; M Nishimura; S Yagyu; M Yoshida; S Akira; J R Downing; T Abe
Journal:  Mol Cell Biol       Date:  2000-01       Impact factor: 4.272

6.  Reciprocal homologous junctions generated in mouse cells.

Authors:  L Desautels; S Brouillette; P Chartrand
Journal:  Mol Gen Genet       Date:  1991-07

7.  Homologous plasmid recombination is elevated in immortally transformed cells.

Authors:  G K Finn; B W Kurz; R Z Cheng; R J Shmookler Reis
Journal:  Mol Cell Biol       Date:  1989-09       Impact factor: 4.272

8.  Mutagenesis in the C-terminal region of human interleukin 5 reveals a central patch for receptor alpha chain recognition.

Authors:  T Morton; J Li; R Cook; I Chaiken
Journal:  Proc Natl Acad Sci U S A       Date:  1995-11-21       Impact factor: 11.205

9.  Human hepatitis delta antigen is a nuclear phosphoprotein with RNA-binding activity.

Authors:  M F Chang; S C Baker; L H Soe; T Kamahora; J G Keck; S Makino; S Govindarajan; M M Lai
Journal:  J Virol       Date:  1988-07       Impact factor: 5.103

10.  Expression cloning of a cDNA for human ceramide glucosyltransferase that catalyzes the first glycosylation step of glycosphingolipid synthesis.

Authors:  S Ichikawa; H Sakiyama; G Suzuki; K I Hidari; Y Hirabayashi
Journal:  Proc Natl Acad Sci U S A       Date:  1996-05-14       Impact factor: 11.205
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